Detailed information of TA system TA072431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1871845..1872461
• Replicon: chromosome
• Accession: NZ_CP019428
• Organism: Pseudomonas sp. R76

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0W0I427
• Locus tag: PspR76_RS08490
• Protein ID: WP_017736377.1
• Coordinates: 1872279..1872461 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PspR76_RS08485
• Protein ID: WP_159954791.1
• Coordinates: 1871845..1872255 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspR76_RS08450	1867409..1867897	-	489	WP_159954785.1	DMT family transporter	-
PspR76_RS08455	1867966..1868889	+	924	WP_159954786.1	LysR family transcriptional regulator	-
PspR76_RS08460	1868908..1869318	+	411	WP_159954787.1	VOC family protein	-
PspR76_RS08465	1869373..1870014	+	642	WP_159954788.1	LysE family translocator	-
PspR76_RS08470	1870134..1870661	+	528	WP_005785958.1	DUF4142 domain-containing protein	-
PspR76_RS08475	1870715..1871140	-	426	WP_159954789.1	low affinity iron permease family protein	-
PspR76_RS08480	1871255..1871755	-	501	WP_159954790.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
PspR76_RS08485	1871845..1872255	-	411	WP_159954791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspR76_RS08490	1872279..1872461	-	183	WP_017736377.1	type II toxin-antitoxin system HicA family toxin	Toxin
PspR76_RS08495	1872629..1873522	-	894	WP_159954792.1	LysR family transcriptional regulator	-
PspR76_RS08500	1873618..1874781	+	1164	WP_159954793.1	MFS transporter	-
PspR76_RS08505	1874778..1875971	+	1194	WP_159954794.1	MFS transporter	-
PspR76_RS08510	1876161..1876475	+	315	WP_159954795.1	hypothetical protein	-
PspR76_RS08515	1876475..1876768	+	294	WP_159954796.1	helix-turn-helix domain-containing protein	-
PspR76_RS08520	1876803..1877108	-	306	WP_159954797.1	metal ABC transporter ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6791.96 Da
• Isoelectric Point: 10.7532

>T72431 WP_017736377.1 NZ_CP019428:c1872461-1872279 [Pseudomonas sp. R76]
VDSRYLIGHIVADGWYLVRTRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T72431 NZ_CP019428:c1872461-1872279 [Pseudomonas sp. R76]
GTGGATAGCCGATATTTGATAGGTCACATCGTTGCGGACGGCTGGTATCTGGTGCGCACCCGAGGCAGTCATCATCACTT
CAAGCACCCGACAAAACCGGGGCTGGTGACGATTCCTCATCCAAAGAAGGACCTGTTGGACAAGACTGCCAAGAGCATTT
TGAAACAGGCCCTGTTGAGCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14811.72 Da
• Isoelectric Point: 4.3129

>AT72431 WP_159954791.1 NZ_CP019428:c1872255-1871845 [Pseudomonas sp. R76]
MLYPIAISTGDEDHAWGVEVPDIPGCYSAGDDLDEAMAMAREAIEGHFEILAEDGAPIPTAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT72431 NZ_CP019428:c1872255-1871845 [Pseudomonas sp. R76]
ATGCTTTACCCCATCGCAATTTCAACCGGTGACGAGGACCACGCCTGGGGCGTGGAAGTCCCGGACATTCCTGGCTGCTA
CTCAGCTGGCGATGACCTGGACGAGGCCATGGCCATGGCCCGGGAGGCCATCGAGGGGCATTTCGAGATCCTCGCGGAAG
ATGGCGCGCCGATTCCCACGGCGCAAAAAGTCACCTTGCACGCAGCGAACCCCCAATACGCCGGCTGCACCTGGGCTGTG
GTGGACATTGATGTCACCAAATACCTGGGCAAAGCGCAGAAGCTCAACATTACCTTGCCGGGTTATCTGTTGAACCGCAT
TGATGAATATGTGCTGAACCACCCGGAAGAAAAAAGTCGTTCGGGGTTTCTGGCGTCCGCTGCGTTGAAAGTATTGCAAC
AGGACCGATGA

Structures

Toxin

• AlphaFold DB: A0A0W0I427