Detailed information of TA system TA072421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1882913..1883529
• Replicon: chromosome
• Accession: NZ_CP019426
• Organism: Pseudomonas sp. R84

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PspR84_RS08515
• Protein ID: WP_160056844.1
• Coordinates: 1882913..1883125 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7Z2KRV3
• Locus tag: PspR84_RS08520
• Protein ID: WP_064390887.1
• Coordinates: 1883125..1883529 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspR84_RS08490	1878088..1880076	+	1989	WP_160056838.1	heme lyase CcmF/NrfE family subunit	-
PspR84_RS08495	1880073..1880609	+	537	WP_007919961.1	DsbE family thiol:disulfide interchange protein	-
PspR84_RS08500	1880606..1881076	+	471	WP_122855443.1	cytochrome c-type biogenesis protein CcmH	-
PspR84_RS08505	1881073..1882275	+	1203	WP_160056840.1	c-type cytochrome biogenesis protein CcmI	-
PspR84_RS08510	1882301..1882705	+	405	WP_160056842.1	hypothetical protein	-
PspR84_RS08515	1882913..1883125	+	213	WP_160056844.1	type II toxin-antitoxin system HicA family toxin	Toxin
PspR84_RS08520	1883125..1883529	+	405	WP_064390887.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspR84_RS08525	1883599..1884786	-	1188	WP_160056846.1	MFS transporter	-
PspR84_RS08530	1885028..1885639	+	612	WP_160056848.1	hypothetical protein	-
PspR84_RS08535	1885880..1886878	-	999	WP_160056850.1	sulfate ABC transporter substrate-binding protein	-
PspR84_RS08540	1886983..1887807	-	825	WP_160056852.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8045.21 Da
• Isoelectric Point: 10.4352

>T72421 WP_160056844.1 NZ_CP019426:1882913-1883125 [Pseudomonas sp. R84]
VQSRLLIKELEEAGWTLDRVTGSHHIFRHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYNPPASYQGDP

Nucleotide

• Length: 213 bp

>T72421 NZ_CP019426:1882913-1883125 [Pseudomonas sp. R84]
GTGCAAAGCAGGCTATTGATCAAGGAGTTGGAGGAGGCAGGCTGGACGCTGGATCGGGTCACTGGCAGTCATCACATCTT
CAGGCACCGATACAACCCGTACACGATTCCCGTTCCTCACCCGAAAAAGGATTTGCCACTGGGGACGGTCAAAAGCATCA
GGAGGCGTGCCGGGTTGTACAACCCGCCAGCCAGCTACCAAGGAGATCCGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14789.75 Da
• Isoelectric Point: 4.3849

>AT72421 WP_064390887.1 NZ_CP019426:1883125-1883529 [Pseudomonas sp. R84]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDSFEDAYKAAVEVAHIMLQEIAADGESIPMPTSASAHRSNPDFADMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT72421 NZ_CP019426:1883125-1883529 [Pseudomonas sp. R84]
ATGCAATATCCAATCTGCATCGAATGGGGTGATGAAAATACCGCCATCGGTATTCAGATCCCTGATATTCCCGGCGCAGT
AACGGCAGGGGATAGCTTTGAAGATGCCTACAAGGCTGCGGTTGAAGTCGCCCACATCATGCTGCAGGAGATTGCGGCGG
ACGGGGAGTCGATTCCTATGCCGACTTCGGCATCCGCTCATCGCAGTAATCCGGACTTTGCCGACATGGGATGGGGCATG
CTCGAGCTGGATATCTCGCCCTATCTCGGCAAGACCGAGAAGGTCAACGTAACGCTGCCCGGTTATGTGATTCAGCGAAT
CGACCGTTATGTGCGTGAACACAACGTTAAAAGTCGCTCCTCCTTTCTGGCGGATGCGGCCATGGAGAAACTGGTTCGTT
ATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z2KRV3