Detailed information of TA system TA072420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1594882..1595502
• Replicon: chromosome
• Accession: NZ_CP019426
• Organism: Pseudomonas sp. R84

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PspR84_RS07205
• Protein ID: WP_160060057.1
• Coordinates: 1595326..1595502 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: J2R7W3
• Locus tag: PspR84_RS07200
• Protein ID: WP_007911473.1
• Coordinates: 1594882..1595289 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspR84_RS07175	1590459..1591229	+	771	WP_160056502.1	trans-aconitate 2-methyltransferase	-
PspR84_RS07180	1591419..1592156	-	738	WP_160056504.1	class I SAM-dependent methyltransferase	-
PspR84_RS07185	1592729..1593091	+	363	WP_160056506.1	hypothetical protein	-
PspR84_RS07190	1593168..1594427	-	1260	WP_160056508.1	type II toxin-antitoxin system HipA family toxin	-
PspR84_RS07195	1594420..1594737	-	318	WP_095119135.1	helix-turn-helix transcriptional regulator	-
PspR84_RS07200	1594882..1595289	-	408	WP_007911473.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspR84_RS07205	1595326..1595502	-	177	WP_160060057.1	type II toxin-antitoxin system HicA family toxin	Toxin
PspR84_RS07210	1595872..1596390	+	519	WP_160060058.1	hypothetical protein	-
PspR84_RS07215	1596346..1596591	-	246	WP_160056510.1	hypothetical protein	-
PspR84_RS07225	1597078..1597962	-	885	WP_077571505.1	alpha/beta hydrolase	-
PspR84_RS07230	1598279..1598971	-	693	WP_095119132.1	pseudouridine synthase	-
PspR84_RS07235	1599005..1599235	-	231	WP_064119704.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-56)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6459.77 Da
• Isoelectric Point: 10.9496

>T72420 WP_160060057.1 NZ_CP019426:c1595502-1595326 [Pseudomonas sp. R84]
IAVFLIGQLVADGWYLVRIKGSHHHFKHPSKPGLVTVPHPKKDLLKKTAISILQQALL

Nucleotide

• Length: 177 bp

>T72420 NZ_CP019426:c1595502-1595326 [Pseudomonas sp. R84]
ATAGCCGTTTTTTTAATCGGCCAACTCGTCGCAGACGGTTGGTATCTGGTAAGGATCAAAGGAAGTCACCATCACTTCAA
GCATCCGAGCAAACCAGGACTGGTCACCGTGCCACATCCAAAAAAGGATCTGCTGAAGAAAACGGCCATCAGTATTTTGC
AGCAGGCGCTGCTTTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14549.58 Da
• Isoelectric Point: 4.7324

>AT72420 WP_007911473.1 NZ_CP019426:c1595289-1594882 [Pseudomonas sp. R84]
MLYPIAISIGDDHHAWGVEVPDIPGCFSAGEDLDDAVAMAREAIEGHFEILADDDAPIPAASKLSLHVANPKYAGCAWAV
VDIDVLKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT72420 NZ_CP019426:c1595289-1594882 [Pseudomonas sp. R84]
ATGCTCTACCCGATTGCGATTTCAATTGGCGATGACCATCACGCCTGGGGTGTAGAAGTCCCGGACATTCCTGGATGCTT
TTCCGCCGGAGAGGATCTGGACGATGCCGTCGCCATGGCTCGCGAAGCCATCGAAGGTCATTTCGAGATTCTGGCGGACG
ACGACGCCCCCATTCCAGCGGCCAGCAAACTGAGCCTGCACGTGGCCAACCCGAAATACGCCGGTTGTGCATGGGCCGTG
GTGGATATCGATGTGCTCAAGTATTTGGGCAAAGCCCAGAAACTCAACATCACTTTGCCGGGGCATTTGCTTAACCGCAT
TGACGAATATGTGTTGCACCACCCGGAAGAAAAAAGCCGCTCCGGCTTTTTGGCGTCGGCGGCTTTGAAAGTTTTGCAGC
AGGGTTGA

Structures

Antitoxin

• AlphaFold DB: J2R7W3