Detailed information of TA system TA072388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 49678..50171
• Replicon: plasmid pXH992_3
• Accession: NZ_CP019393
• Organism: Escherichia coli strain XH992

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: BW707_RS25065
• Protein ID: WP_001230709.1
• Coordinates: 49678..49932 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: BW707_RS25070
• Protein ID: WP_000151456.1
• Coordinates: 49929..50171 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW707_RS25025	45253..45414	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
BW707_RS25030	45563..46159	+	597	WP_000125165.1	hypothetical protein	-
BW707_RS25035	46177..46461	-	285	WP_001162371.1	hypothetical protein	-
BW707_RS25040	46535..47200	-	666	WP_015387337.1	P-loop NTPase	-
BW707_RS25045	47437..47655	+	219	WP_159183630.1	hypothetical protein	-
BW707_RS25050	47787..48116	+	330	WP_000855119.1	hypothetical protein	-
BW707_RS25055	48520..49035	+	516	WP_001025393.1	J domain-containing protein	-
BW707_RS25060	49139..49681	+	543	WP_001083904.1	hypothetical protein	-
BW707_RS25065	49678..49932	+	255	WP_001230709.1	hypothetical protein	Toxin
BW707_RS25070	49929..50171	+	243	WP_000151456.1	hypothetical protein	Antitoxin
BW707_RS25075	50308..50640	+	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
BW707_RS25080	50687..51562	-	876	WP_063859951.1	class A extended-spectrum beta-lactamase CTX-M-132	-
BW707_RS25085	51815..53077	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
BW707_RS25090	53462..53711	+	250	Protein_65	hypothetical protein	-
BW707_RS25095	53827..54114	+	288	WP_000673430.1	hypothetical protein	-
BW707_RS25100	54124..54540	+	417	WP_053882499.1	phage tail protein	-
BW707_RS25105	54537..54848	+	312	WP_001310436.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-132	-	1..63530	63530
	-	flank	IS/Tn	blaCTX-M-132	-	50687..53077	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T72388 WP_001230709.1 NZ_CP019393:49678-49932 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T72388 NZ_CP019393:49678-49932 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT72388 WP_000151456.1 NZ_CP019393:49929-50171 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT72388 NZ_CP019393:49929-50171 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5