Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 4797432..4798138 Replicon chromosome
Accession NZ_CP019392
Organism Escherichia coli strain XH992

Toxin (Protein)


Gene name ypjF Uniprot ID V0SXW1
Locus tag BW707_RS22970 Protein ID WP_000854677.1
Coordinates 4797432..4797800 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID V0SU84
Locus tag BW707_RS22975 Protein ID WP_000065824.1
Coordinates 4797821..4798138 (-) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW707_RS22950 4792812..4794455 + 1644 WP_001265639.1 fimbrial adhesin EcpD -
BW707_RS22955 4794424..4795134 + 711 WP_001361803.1 fimbrial chaperone EcpE -
BW707_RS22960 4795447..4795776 + 330 WP_001303809.1 YkgJ family cysteine cluster protein -
BW707_RS22965 4796025..4796639 - 615 WP_001019920.1 YagU family protein -
BW707_RS22970 4797432..4797800 - 369 WP_000854677.1 TA system toxin CbtA family protein Toxin
BW707_RS22975 4797821..4798138 - 318 WP_000065824.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BW707_RS22980 4798158..4798379 - 222 WP_001220307.1 DUF987 domain-containing protein -
BW707_RS22985 4798388..4798867 - 480 WP_000438147.1 RadC family protein -
BW707_RS22990 4798879..4799337 - 459 WP_000211834.1 antirestriction protein -
BW707_RS22995 4799423..4799659 - 237 Protein_4487 DUF905 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE 4788341..4862652 74311


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-111)

Antitoxin

(5-104)


Sequences


Toxin        


Download         Length: 123 a.a.        Molecular weight: 13687.89 Da        Isoelectric Point: 8.2671

>T72387 WP_000854677.1 NZ_CP019392:c4797800-4797432 [Escherichia coli]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Download         Length: 369 bp

>T72387 NZ_CP019392:c4797800-4797432 [Escherichia coli]
ATGAAAACTCTACCTGCAACGACTCCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTTAC
TCGTCTACTTGAGCAGCACTATGGCCTAATGTTAAGCGACACACCTTTTAGCGATGAAACAGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTTGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTACGACAGACATTCTTCAAGCTAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11964.46 Da        Isoelectric Point: 6.3163

>AT72387 WP_000065824.1 NZ_CP019392:c4798138-4797821 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Download         Length: 318 bp

>AT72387 NZ_CP019392:c4798138-4797821 [Escherichia coli]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACACCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAACT
GCATTATTTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTTACCACTGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
GATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTACTCCACTCAACGCTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SXW1


Antitoxin

Source ID Structure
AlphaFold DB V0SU84

References