Detailed information of TA system TA072387

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4797432..4798138
• Replicon: chromosome
• Accession: NZ_CP019392
• Organism: Escherichia coli strain XH992

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: V0SXW1
• Locus tag: BW707_RS22970
• Protein ID: WP_000854677.1
• Coordinates: 4797432..4797800 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: V0SU84
• Locus tag: BW707_RS22975
• Protein ID: WP_000065824.1
• Coordinates: 4797821..4798138 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW707_RS22950	4792812..4794455	+	1644	WP_001265639.1	fimbrial adhesin EcpD	-
BW707_RS22955	4794424..4795134	+	711	WP_001361803.1	fimbrial chaperone EcpE	-
BW707_RS22960	4795447..4795776	+	330	WP_001303809.1	YkgJ family cysteine cluster protein	-
BW707_RS22965	4796025..4796639	-	615	WP_001019920.1	YagU family protein	-
BW707_RS22970	4797432..4797800	-	369	WP_000854677.1	TA system toxin CbtA family protein	Toxin
BW707_RS22975	4797821..4798138	-	318	WP_000065824.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BW707_RS22980	4798158..4798379	-	222	WP_001220307.1	DUF987 domain-containing protein	-
BW707_RS22985	4798388..4798867	-	480	WP_000438147.1	RadC family protein	-
BW707_RS22990	4798879..4799337	-	459	WP_000211834.1	antirestriction protein	-
BW707_RS22995	4799423..4799659	-	237	Protein_4487	DUF905 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4788341..4862652	74311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.89 Da
• Isoelectric Point: 8.2671

>T72387 WP_000854677.1 NZ_CP019392:c4797800-4797432 [Escherichia coli]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T72387 NZ_CP019392:c4797800-4797432 [Escherichia coli]
ATGAAAACTCTACCTGCAACGACTCCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTTAC
TCGTCTACTTGAGCAGCACTATGGCCTAATGTTAAGCGACACACCTTTTAGCGATGAAACAGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTTGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTACGACAGACATTCTTCAAGCTAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11964.46 Da
• Isoelectric Point: 6.3163

>AT72387 WP_000065824.1 NZ_CP019392:c4798138-4797821 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT72387 NZ_CP019392:c4798138-4797821 [Escherichia coli]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACACCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAACT
GCATTATTTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTTACCACTGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
GATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTACTCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: V0SXW1

Antitoxin

• AlphaFold DB: V0SU84