Detailed information of TA system TA072386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4642407..4643244
• Replicon: chromosome
• Accession: NZ_CP019392
• Organism: Escherichia coli strain XH992

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: BW707_RS22245
• Protein ID: WP_000227787.1
• Coordinates: 4642702..4643244 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: BW707_RS22240
• Protein ID: WP_001353405.1
• Coordinates: 4642407..4642718 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW707_RS22215	4637427..4638374	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
BW707_RS22220	4638396..4640387	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BW707_RS22225	4640377..4640991	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BW707_RS22230	4640991..4641320	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BW707_RS22235	4641332..4642222	+	891	WP_000971336.1	heme o synthase	-
BW707_RS22240	4642407..4642718	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
BW707_RS22245	4642702..4643244	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
BW707_RS22250	4643300..4644235	-	936	WP_012311701.1	sel1 repeat family protein	-
BW707_RS22255	4644642..4646006	+	1365	WP_001000978.1	MFS transporter	-
BW707_RS22260	4646134..4646625	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
BW707_RS22265	4646793..4647704	+	912	WP_000705871.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T72386 WP_000227787.1 NZ_CP019392:4642702-4643244 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T72386 NZ_CP019392:4642702-4643244 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT72386 WP_001353405.1 NZ_CP019392:4642407-4642718 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT72386 NZ_CP019392:4642407-4642718 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGCCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3