Detailed information of TA system TA072375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3347594..3348232
• Replicon: chromosome
• Accession: NZ_CP019392
• Organism: Escherichia coli strain XH992

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: BW707_RS15840
• Protein ID: WP_000813794.1
• Coordinates: 3347594..3347770 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BW707_RS15845
• Protein ID: WP_001270286.1
• Coordinates: 3347816..3348232 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW707_RS15820	3343507..3344427	-	921	Protein_3083	BenE family transporter YdcO	-
BW707_RS15825	3344480..3345016	+	537	WP_000429154.1	DNA-binding transcriptional regulator SutR	-
BW707_RS15830	3345089..3347050	+	1962	WP_012311648.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BW707_RS15835	3347142..3347372	-	231	WP_000491567.1	DUF2554 family protein	-
BW707_RS15840	3347594..3347770	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BW707_RS15845	3347816..3348232	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BW707_RS15850	3348311..3349717	+	1407	WP_000760611.1	PLP-dependent aminotransferase family protein	-
BW707_RS15855	3349962..3351107	+	1146	WP_000034364.1	ABC transporter substrate-binding protein	-
BW707_RS15860	3351125..3352138	+	1014	WP_000220391.1	ABC transporter ATP-binding protein	-
BW707_RS15865	3352139..3353080	+	942	WP_001251303.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3342164..3343492	1328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T72375 WP_000813794.1 NZ_CP019392:3347594-3347770 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T72375 NZ_CP019392:3347594-3347770 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT72375 WP_001270286.1 NZ_CP019392:3347816-3348232 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT72375 NZ_CP019392:3347816-3348232 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• PDB: 6HPB