72370

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3025369..3026153 Replicon chromosome
Accession NZ_CP019392
Organism Escherichia coli strain XH992

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag BW707_RS14295 Protein ID WP_000613626.1
Coordinates 3025369..3025863 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag BW707_RS14300 Protein ID WP_001110446.1
Coordinates 3025860..3026153 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW707_RS14275 3020562..3021659 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
BW707_RS14280 3021659..3022600 + 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
BW707_RS14285 3022666..3024309 + 1644 WP_000096448.1 flagellar hook-associated protein FlgK -
BW707_RS14290 3024321..3025274 + 954 WP_001212763.1 flagellar hook-associated protein FlgL -
BW707_RS14295 3025369..3025863 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
BW707_RS14300 3025860..3026153 - 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
BW707_RS14305 3026287..3029460 - 3174 WP_023141578.1 ribonuclease E -
BW707_RS14310 3030033..3030992 + 960 WP_000846337.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T72370 WP_000613626.1 NZ_CP019392:c3025863-3025369 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T72370 NZ_CP019392:c3025863-3025369 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT72370 WP_001110446.1 NZ_CP019392:c3026153-3025860 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT72370 NZ_CP019392:c3026153-3025860 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References