Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1962531..1963185 Replicon chromosome
Accession NZ_CP019392
Organism Escherichia coli strain XH992

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag BW707_RS09315 Protein ID WP_000244765.1
Coordinates 1962778..1963185 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BW707_RS09310 Protein ID WP_000354046.1
Coordinates 1962531..1962797 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW707_RS09290 1958619..1960052 - 1434 WP_049039059.1 6-phospho-beta-glucosidase BglA -
BW707_RS09295 1960097..1960408 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
BW707_RS09300 1960572..1961231 + 660 WP_000250274.1 hemolysin III family protein -
BW707_RS09305 1961308..1962288 - 981 WP_049038218.1 tRNA-modifying protein YgfZ -
BW707_RS09310 1962531..1962797 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BW707_RS09315 1962778..1963185 + 408 WP_000244765.1 protein YgfX Toxin
BW707_RS09320 1963225..1963746 - 522 WP_001055874.1 flavodoxin FldB -
BW707_RS09325 1963858..1964754 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BW707_RS09330 1964779..1965489 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BW707_RS09335 1965495..1967228 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T72368 WP_000244765.1 NZ_CP019392:1962778-1963185 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T72368 NZ_CP019392:1962778-1963185 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT72368 WP_000354046.1 NZ_CP019392:1962531-1962797 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT72368 NZ_CP019392:1962531-1962797 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References