Detailed information of TA system TA072366

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1732662..1733494
• Replicon: chromosome
• Accession: NZ_CP019392
• Organism: Escherichia coli strain XH992

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: BW707_RS08135
• Protein ID: WP_000854753.1
• Coordinates: 1732662..1733036 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: BW707_RS08140
• Protein ID: WP_001540478.1
• Coordinates: 1733126..1733494 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW707_RS08105	1727775..1728923	-	1149	WP_000905925.1	capsule polysaccharide transporter	-
BW707_RS08110	1728995..1729978	-	984	WP_001355482.1	KpsF/GutQ family sugar-phosphate isomerase	-
BW707_RS08115	1730789..1730959	-	171	Protein_1574	IS110 family transposase	-
BW707_RS08120	1731302..1731871	-	570	WP_001290252.1	DUF4942 domain-containing protein	-
BW707_RS08125	1731968..1732165	-	198	WP_000839281.1	DUF957 domain-containing protein	-
BW707_RS08130	1732177..1732665	-	489	WP_000777545.1	hypothetical protein	-
BW707_RS08135	1732662..1733036	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
BW707_RS08140	1733126..1733494	-	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BW707_RS08145	1733657..1733878	-	222	WP_000692311.1	DUF987 domain-containing protein	-
BW707_RS08150	1733941..1734417	-	477	WP_061092719.1	RadC family protein	-
BW707_RS08155	1734433..1734918	-	486	WP_023909076.1	antirestriction protein	-
BW707_RS08160	1735010..1735828	-	819	WP_001234382.1	DUF945 domain-containing protein	-
BW707_RS08165	1735918..1736151	-	234	WP_001278283.1	DUF905 family protein	-
BW707_RS08170	1736157..1736834	-	678	WP_001097301.1	hypothetical protein	-
BW707_RS08175	1736982..1737662	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T72366 WP_000854753.1 NZ_CP019392:c1733036-1732662 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T72366 NZ_CP019392:c1733036-1732662 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT72366 WP_001540478.1 NZ_CP019392:c1733494-1733126 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT72366 NZ_CP019392:c1733494-1733126 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68