Detailed information of TA system TA072362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 250942..251537
• Replicon: chromosome
• Accession: NZ_CP019392
• Organism: Escherichia coli strain XH992

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: BW707_RS01215
• Protein ID: WP_000239581.1
• Coordinates: 250942..251292 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: BW707_RS01220
• Protein ID: WP_001223213.1
• Coordinates: 251286..251537 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW707_RS01195	246396..247418	-	1023	WP_012311695.1	ABC transporter permease	-
BW707_RS01200	247432..248934	-	1503	WP_001095664.1	sugar ABC transporter ATP-binding protein	-
BW707_RS01205	249067..250023	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
BW707_RS01210	250333..250863	+	531	WP_000055075.1	inorganic diphosphatase	-
BW707_RS01215	250942..251292	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
BW707_RS01220	251286..251537	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
BW707_RS01225	251749..252090	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
BW707_RS01230	252093..255872	-	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T72362 WP_000239581.1 NZ_CP019392:c251292-250942 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T72362 NZ_CP019392:c251292-250942 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT72362 WP_001223213.1 NZ_CP019392:c251537-251286 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT72362 NZ_CP019392:c251537-251286 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7