Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 10650..11238 Replicon chromosome
Accession NZ_CP019388
Organism Winogradskyella sp. J14-2

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag BWZ20_RS00085 Protein ID WP_054560627.1
Coordinates 10936..11238 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag BWZ20_RS00080 Protein ID WP_054560626.1
Coordinates 10650..10934 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWZ20_RS00050 6112..6483 - 372 WP_042249904.1 transposase -
BWZ20_RS00055 6656..7132 - 477 WP_076614678.1 hypothetical protein -
BWZ20_RS00060 7280..7771 - 492 WP_076614683.1 hypothetical protein -
BWZ20_RS00065 7773..8945 - 1173 WP_076614685.1 hypothetical protein -
BWZ20_RS00070 9155..9736 - 582 WP_076614692.1 hypothetical protein -
BWZ20_RS00080 10650..10934 - 285 WP_054560626.1 putative addiction module antidote protein Antitoxin
BWZ20_RS00085 10936..11238 - 303 WP_054560627.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BWZ20_RS00090 11476..12225 - 750 WP_076614696.1 hypothetical protein -
BWZ20_RS00095 12461..13021 - 561 WP_076614699.1 DUF1643 domain-containing protein -
BWZ20_RS00100 13196..13858 - 663 WP_076614701.1 hypothetical protein -
BWZ20_RS00105 14067..15086 - 1020 WP_076614703.1 DUF4238 domain-containing protein -
BWZ20_RS15070 15240..15851 - 612 WP_083677117.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3975..37710 33735


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Uncharacterized protein HI1420

(2-91)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11663.57 Da        Isoelectric Point: 10.3063

>T72352 WP_054560627.1 NZ_CP019388:c11238-10936 [Winogradskyella sp. J14-2]
MIRTEKTSEFDKWISKLKDLRAKAKILFRIQKLESDGHFGDCKPVGDGISEMRINYAKGYRVYFKEKGNQIVVLLIGGDK
STQQKDIKKAKEIWNRIKDE

Download         Length: 303 bp

>T72352 NZ_CP019388:c11238-10936 [Winogradskyella sp. J14-2]
ATGATAAGAACCGAAAAGACATCCGAATTTGACAAGTGGATTAGTAAGCTGAAAGATTTAAGAGCGAAAGCAAAAATCCT
CTTCCGAATTCAGAAACTCGAATCGGATGGACATTTTGGAGACTGTAAACCTGTTGGAGATGGAATAAGCGAAATGCGAA
TTAATTACGCCAAAGGTTACAGAGTATATTTCAAAGAAAAAGGAAACCAAATTGTTGTTCTTTTGATTGGTGGAGATAAA
TCGACCCAACAAAAGGACATCAAAAAAGCGAAAGAAATCTGGAATAGAATAAAAGACGAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10047.60 Da        Isoelectric Point: 4.7045

>AT72352 WP_054560626.1 NZ_CP019388:c10934-10650 [Winogradskyella sp. J14-2]
MATTKFDIADYLDSEEMIAEYLNTVLEDGDSSDLIVAIGHIAKAIGMTKIAEQTGMSRPSLYKALSDGAKPQFGTIMKVL
KAIGGQINVRPISA

Download         Length: 285 bp

>AT72352 NZ_CP019388:c10934-10650 [Winogradskyella sp. J14-2]
ATGGCGACAACAAAATTTGACATAGCGGATTATTTGGACAGCGAAGAAATGATTGCCGAATACTTGAATACGGTTCTTGA
AGACGGAGACAGTTCTGATTTGATTGTGGCAATCGGTCACATTGCAAAGGCAATCGGAATGACCAAAATAGCGGAACAAA
CTGGAATGAGCAGACCAAGCCTCTACAAAGCATTATCGGATGGTGCAAAACCACAGTTTGGAACCATAATGAAAGTGCTG
AAAGCAATTGGCGGACAGATAAACGTAAGACCAATATCAGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References