Detailed information of TA system TA072348

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3628832..3629667
• Replicon: chromosome
• Accession: NZ_CP019361
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: BW158_RS19590
• Protein ID: WP_000854821.1
• Coordinates: 3628832..3629209 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BW158_RS19595
• Protein ID: WP_203170283.1
• Coordinates: 3629299..3629667 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS19560	3623941..3624252	-	312	WP_001436958.1	hypothetical protein	-
BW158_RS19565	3625097..3625249	-	153	WP_000788776.1	hypothetical protein	-
BW158_RS19570	3626068..3627387	+	1320	WP_000144688.1	site-specific integrase	-
BW158_RS19575	3627480..3628328	-	849	WP_001280470.1	DUF4942 domain-containing protein	-
BW158_RS19580	3628413..3628610	-	198	WP_089075429.1	DUF957 domain-containing protein	-
BW158_RS19585	3628638..3628835	-	198	Protein_3470	hypothetical protein	-
BW158_RS19590	3628832..3629209	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BW158_RS19595	3629299..3629667	-	369	WP_203170283.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BW158_RS19600	3629747..3629968	-	222	WP_000692350.1	DUF987 domain-containing protein	-
BW158_RS19605	3630055..3630531	-	477	WP_001186745.1	RadC family protein	-
BW158_RS19610	3630547..3631032	-	486	WP_024214936.1	antirestriction protein	-
BW158_RS19615	3631124..3631942	-	819	WP_001234397.1	DUF945 domain-containing protein	-
BW158_RS19620	3632032..3632265	-	234	WP_001117566.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3587009..3642024	55015

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T72348 WP_000854821.1 NZ_CP019361:c3629209-3628832 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T72348 NZ_CP019361:c3629209-3628832 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13620.38 Da
• Isoelectric Point: 6.4761

>AT72348 WP_203170283.1 NZ_CP019361:c3629667-3629299 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSNADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT72348 NZ_CP019361:c3629667-3629299 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCAACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54