Detailed information of TA system TA072347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3468324..3469161
• Replicon: chromosome
• Accession: NZ_CP019361
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: BW158_RS18790
• Protein ID: WP_000227786.1
• Coordinates: 3468619..3469161 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BW158_RS18785
• Protein ID: WP_001297137.1
• Coordinates: 3468324..3468635 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS18760	3463344..3464291	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
BW158_RS18765	3464313..3466304	+	1992	WP_113403942.1	cytochrome o ubiquinol oxidase subunit I	-
BW158_RS18770	3466294..3466908	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BW158_RS18775	3466908..3467237	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BW158_RS18780	3467249..3468139	+	891	WP_000971336.1	heme o synthase	-
BW158_RS18785	3468324..3468635	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BW158_RS18790	3468619..3469161	+	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
BW158_RS18795	3469217..3470152	-	936	WP_001297127.1	sel1 repeat family protein	-
BW158_RS18800	3470569..3471933	+	1365	WP_001000978.1	MFS transporter	-
BW158_RS18805	3472061..3472552	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
BW158_RS18810	3472720..3473631	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T72347 WP_000227786.1 NZ_CP019361:3468619-3469161 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T72347 NZ_CP019361:3468619-3469161 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT72347 WP_001297137.1 NZ_CP019361:3468324-3468635 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT72347 NZ_CP019361:3468324-3468635 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3