Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2447965..2448603 Replicon chromosome
Accession NZ_CP019361
Organism Escherichia coli strain XH993

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BW158_RS13775 Protein ID WP_000813794.1
Coordinates 2448427..2448603 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BW158_RS13770 Protein ID WP_001270286.1
Coordinates 2447965..2448381 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW158_RS13750 2443117..2444058 - 942 WP_001251313.1 ABC transporter permease -
BW158_RS13755 2444059..2445072 - 1014 WP_000220415.1 ABC transporter ATP-binding protein -
BW158_RS13760 2445090..2446235 - 1146 WP_113403949.1 ABC transporter substrate-binding protein -
BW158_RS13765 2446480..2447886 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
BW158_RS13770 2447965..2448381 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BW158_RS13775 2448427..2448603 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BW158_RS13780 2448825..2449055 + 231 WP_000494244.1 YncJ family protein -
BW158_RS13785 2449147..2451108 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BW158_RS13790 2451181..2451717 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
BW158_RS13795 2451770..2452984 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T72345 WP_000813794.1 NZ_CP019361:c2448603-2448427 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T72345 NZ_CP019361:c2448603-2448427 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT72345 WP_001270286.1 NZ_CP019361:c2448381-2447965 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT72345 NZ_CP019361:c2448381-2447965 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References