Detailed information of TA system TA072343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2304427..2304953
• Replicon: chromosome
• Accession: NZ_CP019361
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: BW158_RS13055
• Protein ID: WP_000323025.1
• Coordinates: 2304666..2304953 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: BW158_RS13050
• Protein ID: WP_000534858.1
• Coordinates: 2304427..2304666 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS13030	2300267..2301636	+	1370	WP_168728556.1	IS3-like element IS150 family transposase	-
BW158_RS13035	2301812..2303125	+	1314	WP_000589005.1	ISNCY family transposase	-
BW158_RS13040	2303562..2303894	-	333	WP_001301033.1	protein FlxA	-
BW158_RS13045	2304097..2304402	-	306	WP_071598992.1	hypothetical protein	-
BW158_RS13050	2304427..2304666	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
BW158_RS13055	2304666..2304953	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
BW158_RS13060	2305025..2305180	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
BW158_RS13065	2305397..2305648	+	252	WP_000980994.1	hypothetical protein	-
BW158_RS13070	2305874..2306035	+	162	Protein_2190	antitermination protein	-
BW158_RS13075	2306313..2306402	-	90	WP_120795389.1	hypothetical protein	-
BW158_RS13080	2306457..2306669	-	213	WP_000087756.1	cold shock-like protein CspF	-
BW158_RS13085	2306970..2307185	+	216	WP_000066484.1	cold shock-like protein CspB	-
BW158_RS13090	2307939..2308154	+	216	WP_000839590.1	phage lysis protein EssD	-
BW158_RS13095	2308159..2308470	+	312	WP_000189916.1	YdfR family protein	-
BW158_RS13100	2308467..2309000	+	534	WP_001092971.1	lysozyme	-
BW158_RS13105	2308997..2309494	+	498	WP_001071769.1	DUF2514 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2267418..2335767	68349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T72343 WP_000323025.1 NZ_CP019361:2304666-2304953 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T72343 NZ_CP019361:2304666-2304953 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT72343 WP_000534858.1 NZ_CP019361:2304427-2304666 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT72343 NZ_CP019361:2304427-2304666 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8