Detailed information of TA system TA072331

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 790707..791542
• Replicon: chromosome
• Accession: NZ_CP019361
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BW158_RS05660
• Protein ID: WP_057109034.1
• Coordinates: 790707..791084 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: BW158_RS05665
• Protein ID: WP_001285610.1
• Coordinates: 791174..791542 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS05630	786712..787208	+	497	Protein_736	type VI secretion system contractile sheath small subunit	-
BW158_RS05635	787742..788035	+	294	WP_001110459.1	DUF1778 domain-containing protein	-
BW158_RS05640	788032..788520	+	489	WP_000622309.1	GNAT family N-acetyltransferase	-
BW158_RS05645	789361..790203	-	843	WP_039004748.1	DUF4942 domain-containing protein	-
BW158_RS05650	790288..790485	-	198	WP_023563519.1	DUF957 domain-containing protein	-
BW158_RS05655	790561..790710	-	150	Protein_741	hypothetical protein	-
BW158_RS05660	790707..791084	-	378	WP_057109034.1	TA system toxin CbtA family protein	Toxin
BW158_RS05665	791174..791542	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BW158_RS05670	791616..791837	-	222	Protein_744	DUF987 domain-containing protein	-
BW158_RS05675	791924..792400	-	477	WP_057109035.1	RadC family protein	-
BW158_RS05680	792416..792895	-	480	WP_057109036.1	antirestriction protein	-
BW158_RS05685	792995..793135	+	141	WP_000997937.1	hypothetical protein	-
BW158_RS05690	793161..793979	-	819	WP_001175153.1	DUF945 domain-containing protein	-
BW158_RS05695	794069..794302	-	234	WP_001278287.1	DUF905 family protein	-
BW158_RS05700	794308..794985	-	678	WP_001097368.1	hypothetical protein	-
BW158_RS05705	795104..795988	-	885	WP_000010392.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13934.86 Da
• Isoelectric Point: 6.8517

>T72331 WP_057109034.1 NZ_CP019361:c791084-790707 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T72331 NZ_CP019361:c791084-790707 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT72331 WP_001285610.1 NZ_CP019361:c791542-791174 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT72331 NZ_CP019361:c791542-791174 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA