Detailed information of TA system TA072330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 787742..788520
• Replicon: chromosome
• Accession: NZ_CP019361
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: BW158_RS05640
• Protein ID: WP_000622309.1
• Coordinates: 788032..788520 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7H9KCB5
• Locus tag: BW158_RS05635
• Protein ID: WP_001110459.1
• Coordinates: 787742..788035 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS05615	783506..784489	-	984	WP_162753665.1	KpsF/GutQ family sugar-phosphate isomerase	-
BW158_RS05620	785314..785649	-	336	WP_000789899.1	hypothetical protein	-
BW158_RS05625	785646..786119	-	474	WP_000143249.1	hypothetical protein	-
BW158_RS05630	786712..787208	+	497	Protein_736	type VI secretion system contractile sheath small subunit	-
BW158_RS05635	787742..788035	+	294	WP_001110459.1	DUF1778 domain-containing protein	Antitoxin
BW158_RS05640	788032..788520	+	489	WP_000622309.1	GNAT family N-acetyltransferase	Toxin
BW158_RS05645	789361..790203	-	843	WP_039004748.1	DUF4942 domain-containing protein	-
BW158_RS05650	790288..790485	-	198	WP_023563519.1	DUF957 domain-containing protein	-
BW158_RS05655	790561..790710	-	150	Protein_741	hypothetical protein	-
BW158_RS05660	790707..791084	-	378	WP_057109034.1	TA system toxin CbtA family protein	-
BW158_RS05665	791174..791542	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	-
BW158_RS05670	791616..791837	-	222	Protein_744	DUF987 domain-containing protein	-
BW158_RS05675	791924..792400	-	477	WP_057109035.1	RadC family protein	-
BW158_RS05680	792416..792895	-	480	WP_057109036.1	antirestriction protein	-
BW158_RS05685	792995..793135	+	141	WP_000997937.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (83-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17491.33 Da
• Isoelectric Point: 7.7535

>T72330 WP_000622309.1 NZ_CP019361:788032-788520 [Escherichia coli]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVTTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T72330 NZ_CP019361:788032-788520 [Escherichia coli]
ATGATCTCCGCTCCTGAGCCGCTTCACGCCGGGCATATTCTTACCCCATTTTGCTGTGGCGTGGATTCTATGGATAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGTACTTTTGTATGTTGCGGCAGTGATTCTAAGGTAC
TGGCGTATTACTCGCTGGCATCCAGTGCAGTGACGACAAACACTGCCCCCGGCCGCTTTCGCCGTAATATGCCCGATCCG
ATCCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATCAGTCTATACATGGGCAGGGTGTTGGTCGGGCGCTGGTACGTGA
CGCCGGGCTGCGGGTGATTCAGGTGGCTGAAACTATTGGTATTCGCGGGATGCTGGTTCATGCGCTGTCTGATGAGGCGC
GGGATTTCTATCTGCGGGTGGGGTTTGAGCCGTCACCGATGGATCCGATGATGTTGCTGGTGACGTTGGGGGATTTGGTT
CATGCTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 9.9171

>AT72330 WP_001110459.1 NZ_CP019361:787742-788035 [Escherichia coli]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT72330 NZ_CP019361:787742-788035 [Escherichia coli]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAATCTGCGCATCAGGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCGAAAGCCAGAGGAAAAAACCGCACTGACTTTGTGCTGGAGGCCGCCCGCGCCGCGGCGGAAGAGGCGCTGA
TCGAGCAGCGTATCATTATGGCCGATCCGGAGGCTTATCAGACGTTTCTTACCCGCCTTGATCAGGCTCCAGCGCCAAAC
TCCGCGCTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7H9KCB5