Detailed information of TA system TA072323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 183342..183967
• Replicon: plasmid pXH993
• Accession: NZ_CP019360
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BW158_RS01000
• Protein ID: WP_000911313.1
• Coordinates: 183342..183740 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: BW158_RS01005
• Protein ID: WP_000450520.1
• Coordinates: 183740..183967 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS00965	178676..180103	+	1428	WP_000146685.1	conjugal transfer protein TraB	-
BW158_RS00970	180093..180683	+	591	WP_001376242.1	conjugal transfer protein TraP	-
BW158_RS00975	180637..180867	+	231	WP_001352845.1	conjugal transfer protein TrbD	-
BW158_RS00980	180879..181130	+	252	WP_001038342.1	conjugal transfer protein TrbG	-
BW158_RS00985	181127..181642	+	516	WP_000809838.1	type IV conjugative transfer system lipoprotein TraV	-
BW158_RS00990	181777..181998	+	222	WP_001278689.1	conjugal transfer protein TraR	-
BW158_RS00995	182287..183333	+	1047	Protein_198	type IV secretion system DNA-binding domain-containing protein	-
BW158_RS01000	183342..183740	-	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BW158_RS01005	183740..183967	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA / aac(3)-IId / sitABCD / mph(A) / aph(3')-Ia / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 / mcr-1.1	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..349248	349248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T72323 WP_000911313.1 NZ_CP019360:c183740-183342 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T72323 NZ_CP019360:c183740-183342 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT72323 WP_000450520.1 NZ_CP019360:c183967-183740 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT72323 NZ_CP019360:c183967-183740 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2