Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4386191..4386688 Replicon chromosome
Accession NZ_CP019359
Organism Escherichia coli strain XH990

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag BW242_RS23230 Protein ID WP_001672275.1
Coordinates 4386191..4386364 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BW242_RS23235 Protein ID WP_000398619.1
Coordinates 4386392..4386688 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW242_RS23220 4382861..4384318 + 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -
BW242_RS23225 4384555..4386072 + 1518 WP_001295074.1 lysine--tRNA ligase -
BW242_RS23230 4386191..4386364 - 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
BW242_RS23235 4386392..4386688 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BW242_RS23240 4386915..4387187 - 273 WP_000405647.1 N-acetyltransferase -
BW242_RS23245 4387199..4387429 - 231 WP_021557455.1 (4Fe-4S)-binding protein -
BW242_RS23250 4387610..4389241 + 1632 WP_001216477.1 sensor histidine kinase -
BW242_RS23255 4389238..4389957 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T72317 WP_001672275.1 NZ_CP019359:c4386364-4386191 [Escherichia coli]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T72317 NZ_CP019359:c4386364-4386191 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT72317 WP_000398619.1 NZ_CP019359:c4386688-4386392 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT72317 NZ_CP019359:c4386688-4386392 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References