Detailed information of TA system TA072315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4077398..4077948
• Replicon: chromosome
• Accession: NZ_CP019359
• Organism: Escherichia coli strain XH990

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: BW242_RS21780
• Protein ID: WP_001160975.1
• Coordinates: 4077398..4077712 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: BW242_RS21785
• Protein ID: WP_000125565.1
• Coordinates: 4077715..4077948 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW242_RS21755	4073073..4074359	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
BW242_RS21760	4074359..4075348	+	990	WP_021556398.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
BW242_RS21765	4075345..4076166	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
BW242_RS21770	4076169..4076546	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
BW242_RS21775	4076553..4077401	+	849	WP_021556397.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
BW242_RS21780	4077398..4077712	-	315	WP_001160975.1	CcdB family protein	Toxin
BW242_RS21785	4077715..4077948	-	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
BW242_RS21790	4078034..4078513	-	480	WP_001487721.1	type 3 dihydrofolate reductase	-
BW242_RS21795	4078705..4080567	-	1863	WP_040234481.1	glutathione-regulated potassium-efflux system protein KefC	-
BW242_RS21800	4080560..4081090	-	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
BW242_RS21805	4081201..4082532	-	1332	WP_001183198.1	MFS transporter	-
BW242_RS21810	4082590..4082877	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T72315 WP_001160975.1 NZ_CP019359:c4077712-4077398 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T72315 NZ_CP019359:c4077712-4077398 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT72315 WP_000125565.1 NZ_CP019359:c4077948-4077715 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT72315 NZ_CP019359:c4077948-4077715 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7