Detailed information of TA system TA072286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3558641..3559478
• Replicon: chromosome
• Accession: NZ_CP019355
• Organism: Escherichia coli strain XH988

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: BWZ24_RS19220
• Protein ID: WP_000227784.1
• Coordinates: 3558936..3559478 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BWZ24_RS19215
• Protein ID: WP_001297137.1
• Coordinates: 3558641..3558952 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWZ24_RS19190	3553661..3554608	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
BWZ24_RS19195	3554630..3556621	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BWZ24_RS19200	3556611..3557225	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BWZ24_RS19205	3557225..3557554	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BWZ24_RS19210	3557566..3558456	+	891	WP_000971336.1	heme o synthase	-
BWZ24_RS19215	3558641..3558952	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BWZ24_RS19220	3558936..3559478	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
BWZ24_RS19225	3559534..3560469	-	936	WP_001297127.1	sel1 repeat family protein	-
BWZ24_RS19230	3560877..3562241	+	1365	WP_001000978.1	MFS transporter	-
BWZ24_RS19235	3562369..3562860	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
BWZ24_RS19240	3563028..3563939	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T72286 WP_000227784.1 NZ_CP019355:3558936-3559478 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T72286 NZ_CP019355:3558936-3559478 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT72286 WP_001297137.1 NZ_CP019355:3558641-3558952 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT72286 NZ_CP019355:3558641-3558952 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3