Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1317671..1318296 Replicon chromosome
Accession NZ_CP019355
Organism Escherichia coli strain XH988

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag BWZ24_RS08285 Protein ID WP_000911329.1
Coordinates 1317898..1318296 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag BWZ24_RS08280 Protein ID WP_000450524.1
Coordinates 1317671..1317898 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWZ24_RS08255 1313474..1313944 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
BWZ24_RS08260 1313944..1314516 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
BWZ24_RS08265 1314662..1315540 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
BWZ24_RS08270 1315557..1316591 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
BWZ24_RS08275 1316804..1317517 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
BWZ24_RS08280 1317671..1317898 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
BWZ24_RS08285 1317898..1318296 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
BWZ24_RS08290 1318443..1319306 + 864 WP_001267498.1 neutral zinc metallopeptidase -
BWZ24_RS08295 1319321..1321336 + 2016 WP_032254558.1 tRNA cytosine(34) acetyltransferase TmcA -
BWZ24_RS08300 1321410..1322108 + 699 WP_000679823.1 esterase -
BWZ24_RS08305 1322218..1322418 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T72275 WP_000911329.1 NZ_CP019355:1317898-1318296 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T72275 NZ_CP019355:1317898-1318296 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT72275 WP_000450524.1 NZ_CP019355:1317671-1317898 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT72275 NZ_CP019355:1317671-1317898 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References