Detailed information of TA system TA072270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 6545..7343
• Replicon: plasmid pXH988_2
• Accession: NZ_CP019354
• Organism: Escherichia coli strain XH988

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: BWZ24_RS01415
• Protein ID: WP_203163683.1
• Coordinates: 6545..7066 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: BWZ24_RS01420
• Protein ID: WP_001711191.1
• Coordinates: 7074..7343 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWZ24_RS01395	4234..5019	+	786	WP_203163682.1	receptor-recognizing protein	-
BWZ24_RS01400	5094..5417	+	324	WP_032084070.1	hypothetical protein	-
BWZ24_RS01405	5431..6123	+	693	WP_032084069.1	membrane protein	-
BWZ24_RS01410	6125..6376	+	252	WP_000901559.1	hypothetical protein	-
BWZ24_RS01415	6545..7066	-	522	WP_203163683.1	GNAT family N-acetyltransferase	Toxin
BWZ24_RS01420	7074..7343	-	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
BWZ24_RS01425	7663..8331	+	669	WP_086722033.1	AAA family ATPase	-
BWZ24_RS01430	8337..8690	+	354	WP_160378290.1	hypothetical protein	-
BWZ24_RS01435	8743..9510	-	768	WP_000342417.1	hypothetical protein	-
BWZ24_RS01440	9795..10535	+	741	WP_203163684.1	hypothetical protein	-
BWZ24_RS01445	10579..11919	+	1341	WP_000137333.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107661	107661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19340.13 Da
• Isoelectric Point: 7.8107

>T72270 WP_203163683.1 NZ_CP019354:c7066-6545 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGHGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T72270 NZ_CP019354:c7066-6545 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAACGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCATGGGCT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT72270 WP_001711191.1 NZ_CP019354:c7343-7074 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT72270 NZ_CP019354:c7343-7074 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2