Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 2067022..2067610 Replicon chromosome
Accession NZ_CP019347
Organism Brucella sp. 09RB8471

Toxin (Protein)


Gene name PumA Uniprot ID A0A7L7ML11
Locus tag BKD03_RS16675 Protein ID WP_008505629.1
Coordinates 2067320..2067610 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7L7ML36
Locus tag BKD03_RS16670 Protein ID WP_008505633.1
Coordinates 2067022..2067318 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKD03_RS16620 2062430..2062684 + 255 WP_076781332.1 hypothetical protein -
BKD03_RS16625 2062685..2062999 - 315 WP_002964097.1 BrnA antitoxin family protein -
BKD03_RS16630 2062999..2063244 - 246 WP_004688321.1 BrnT family toxin -
BKD03_RS16635 2063331..2063588 + 258 WP_076781333.1 hypothetical protein -
BKD03_RS16640 2064264..2064491 + 228 WP_076781335.1 hypothetical protein -
BKD03_RS16645 2064507..2064701 + 195 WP_076781338.1 hypothetical protein -
BKD03_RS16650 2064749..2065243 + 495 WP_076781341.1 hypothetical protein -
BKD03_RS16655 2065262..2065651 + 390 WP_076781343.1 hypothetical protein -
BKD03_RS16665 2066046..2066933 - 888 WP_154142821.1 hypothetical protein -
BKD03_RS16670 2067022..2067318 - 297 WP_008505633.1 putative addiction module antidote protein Antitoxin
BKD03_RS16675 2067320..2067610 - 291 WP_008505629.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BKD03_RS16680 2067730..2067990 - 261 WP_083698679.1 Arc family DNA-binding protein -
BKD03_RS17435 2068097..2068270 + 174 WP_083698680.1 hypothetical protein -
BKD03_RS16690 2068408..2068668 + 261 WP_076781352.1 hypothetical protein -
BKD03_RS16705 2069491..2069742 - 252 WP_076781353.1 GlsB/YeaQ/YmgE family stress response membrane protein -
BKD03_RS16715 2070210..2071235 - 1026 WP_076781356.1 site-specific integrase -
BKD03_RS16720 2071266..2071550 + 285 WP_154142823.1 hypothetical protein -
BKD03_RS16725 2071587..2071934 + 348 WP_076781360.1 arsenate reductase (glutaredoxin) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2058230..2076676 18446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-95)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10724.46 Da        Isoelectric Point: 10.2779

>T72268 WP_008505629.1 NZ_CP019347:c2067610-2067320 [Brucella sp. 09RB8471]
MIEVRQTTAFTKWLNELRDTNARLRIATRIRRMELGNPGDVKAVGEGVSEMRITYGPGYRVYFVTMGSTIVVLLCGGDKS
SQSRDIAVAKQMAKEI

Download         Length: 291 bp

>T72268 NZ_CP019347:c2067610-2067320 [Brucella sp. 09RB8471]
ATGATCGAAGTTCGCCAAACCACAGCATTCACAAAATGGCTCAATGAGCTTCGAGACACCAATGCGCGTTTAAGGATTGC
CACTCGTATCCGACGAATGGAGCTTGGCAATCCAGGTGATGTGAAAGCCGTTGGTGAAGGTGTGAGCGAGATGCGTATTA
CCTATGGCCCCGGCTATCGGGTTTATTTCGTGACCATGGGTTCAACCATTGTCGTTCTGTTATGTGGCGGTGACAAGTCA
TCACAAAGCCGGGATATTGCCGTGGCGAAACAAATGGCAAAGGAGATTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10440.88 Da        Isoelectric Point: 5.1057

>AT72268 WP_008505633.1 NZ_CP019347:c2067318-2067022 [Brucella sp. 09RB8471]
MALETTRFDILDHLKTPEERLAYLEAAFEDGDPSLIAHALGDVARSVGMTAVAKEAGITREALYRSLSENGDPKLSTLLG
VTKALGIHLTVGPDKHAA

Download         Length: 297 bp

>AT72268 NZ_CP019347:c2067318-2067022 [Brucella sp. 09RB8471]
ATGGCCCTTGAAACCACTCGTTTCGATATTCTCGACCATCTCAAGACGCCGGAGGAGCGCCTTGCTTATCTCGAAGCAGC
ATTTGAGGATGGCGACCCTTCTCTTATCGCTCATGCCCTTGGCGATGTGGCCCGCTCCGTTGGTATGACTGCCGTTGCCA
AAGAAGCAGGCATTACCCGTGAGGCGCTTTACAGATCGCTCAGCGAAAACGGCGACCCGAAACTATCGACCTTGCTTGGC
GTTACCAAAGCCCTCGGCATTCATTTGACAGTCGGGCCAGACAAACACGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L7ML11

Antitoxin

Source ID Structure
AlphaFold DB A0A7L7ML36

References