Detailed information of TA system TA072267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2062685..2063244
• Replicon: chromosome
• Accession: NZ_CP019347
• Organism: Brucella sp. 09RB8471

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BKD03_RS16630
• Protein ID: WP_004688321.1
• Coordinates: 2062999..2063244 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BKD03_RS16625
• Protein ID: WP_002964097.1
• Coordinates: 2062685..2062999 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKD03_RS16580	2058230..2059237	-	1008	WP_076781320.1	site-specific integrase	-
BKD03_RS16585	2059410..2059625	-	216	WP_076781322.1	hypothetical protein	-
BKD03_RS16590	2059622..2059825	-	204	WP_076781324.1	hypothetical protein	-
BKD03_RS16595	2059822..2060202	-	381	WP_076781326.1	hypothetical protein	-
BKD03_RS16600	2060202..2060540	-	339	WP_076781329.1	hypothetical protein	-
BKD03_RS16605	2060819..2061399	+	581	Protein_1963	hypothetical protein	-
BKD03_RS16620	2062430..2062684	+	255	WP_076781332.1	hypothetical protein	-
BKD03_RS16625	2062685..2062999	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BKD03_RS16630	2062999..2063244	-	246	WP_004688321.1	BrnT family toxin	Toxin
BKD03_RS16635	2063331..2063588	+	258	WP_076781333.1	hypothetical protein	-
BKD03_RS16640	2064264..2064491	+	228	WP_076781335.1	hypothetical protein	-
BKD03_RS16645	2064507..2064701	+	195	WP_076781338.1	hypothetical protein	-
BKD03_RS16650	2064749..2065243	+	495	WP_076781341.1	hypothetical protein	-
BKD03_RS16655	2065262..2065651	+	390	WP_076781343.1	hypothetical protein	-
BKD03_RS16665	2066046..2066933	-	888	WP_154142821.1	hypothetical protein	-
BKD03_RS16670	2067022..2067318	-	297	WP_008505633.1	putative addiction module antidote protein	-
BKD03_RS16675	2067320..2067610	-	291	WP_008505629.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BKD03_RS16680	2067730..2067990	-	261	WP_083698679.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2058230..2076676	18446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T72267 WP_004688321.1 NZ_CP019347:c2063244-2062999 [Brucella sp. 09RB8471]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T72267 NZ_CP019347:c2063244-2062999 [Brucella sp. 09RB8471]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT72267 WP_002964097.1 NZ_CP019347:c2062999-2062685 [Brucella sp. 09RB8471]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT72267 NZ_CP019347:c2062999-2062685 [Brucella sp. 09RB8471]
ATGGGTATCAAATTTTCCTCTAAGCGTCCTCTCACTCAGGAAGAGGAAGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGACTTTGA