Detailed information of TA system TA072266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 168346..169016
• Replicon: chromosome
• Accession: NZ_CP019347
• Organism: Brucella sp. 09RB8471

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BKD03_RS06805
• Protein ID: WP_076770566.1
• Coordinates: 168346..168750 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D1CXH4
• Locus tag: BKD03_RS06810
• Protein ID: WP_008504606.1
• Coordinates: 168747..169016 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKD03_RS06775	164013..164249	+	237	WP_008505519.1	BolA family transcriptional regulator	-
BKD03_RS06780	164431..164763	+	333	WP_009364410.1	Grx4 family monothiol glutaredoxin	-
BKD03_RS06790	165034..166299	+	1266	WP_076779580.1	multidrug effflux MFS transporter	-
BKD03_RS06795	166353..167180	-	828	WP_076779582.1	inositol monophosphatase family protein	-
BKD03_RS06800	167167..168047	-	881	Protein_158	tRNA 2-thiocytidine(32) synthetase TtcA	-
BKD03_RS06805	168346..168750	-	405	WP_076770566.1	type II toxin-antitoxin system VapC family toxin	Toxin
BKD03_RS06810	168747..169016	-	270	WP_008504606.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BKD03_RS06815	169148..169765	-	618	WP_076779584.1	30S ribosomal protein S4	-
BKD03_RS06820	170100..171071	+	972	WP_076779586.1	extensin family protein	-
BKD03_RS06825	171343..171756	+	414	WP_008935646.1	hypothetical protein	-
BKD03_RS06830	171801..171998	-	198	WP_002963959.1	DUF3008 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14305.62 Da
• Isoelectric Point: 5.6357

>T72266 WP_076770566.1 NZ_CP019347:c168750-168346 [Brucella sp. 09RB8471]
MSKLYMLDTNIVSDLARNPHGPVAKHIARVGADAICVSIITACELRYGCAKKGSPRLLAQIEAILDSIEILPLDVPADTE
YGGIRAELEADGKPIGPNDFLIAAHACALGAVLVTANVGEFTRIRKLAVENWLN

Nucleotide

• Length: 405 bp

>T72266 NZ_CP019347:c168750-168346 [Brucella sp. 09RB8471]
TTGAGCAAGCTTTACATGCTGGACACCAACATCGTGTCGGATCTTGCTCGTAACCCTCATGGCCCTGTGGCAAAACATAT
CGCTCGCGTGGGGGCTGATGCGATTTGCGTAAGCATCATTACCGCCTGCGAACTGCGTTATGGTTGTGCGAAAAAGGGTT
CGCCAAGACTGCTCGCACAGATAGAGGCAATACTGGATAGCATTGAAATTCTGCCGCTCGATGTTCCTGCCGATACGGAG
TATGGGGGCATACGGGCAGAACTGGAAGCTGATGGAAAGCCTATCGGTCCCAATGACTTTCTTATCGCGGCTCACGCTTG
CGCACTCGGTGCGGTTCTGGTTACGGCCAATGTCGGTGAGTTTACCCGCATCCGTAAACTTGCTGTCGAAAACTGGTTGA
ATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10046.62 Da
• Isoelectric Point: 7.2059

>AT72266 WP_008504606.1 NZ_CP019347:c169016-168747 [Brucella sp. 09RB8471]
MAHARRIEPTPAREAKLFRNNKSQAVRIPADFEFPGNRVLIHREGDKLIIEPVRRKNLLEVLAGLKPLGPEDAFPDIDDT
ALPLKDVDL

Nucleotide

• Length: 270 bp

>AT72266 NZ_CP019347:c169016-168747 [Brucella sp. 09RB8471]
ATGGCACACGCAAGACGTATCGAGCCAACGCCTGCGCGCGAGGCAAAGCTTTTTCGCAACAATAAGAGTCAGGCAGTTCG
TATTCCGGCTGATTTTGAGTTTCCGGGAAACCGTGTGCTTATCCATCGTGAAGGGGACAAGCTCATCATCGAACCTGTTC
GCCGAAAGAATCTGCTCGAAGTTCTGGCAGGATTAAAGCCACTTGGACCAGAGGACGCTTTCCCGGACATTGATGATACA
GCCCTTCCGCTTAAGGATGTAGACCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7L7MKG1