Detailed information of TA system TA072250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5855824..5857438
• Replicon: chromosome
• Accession: NZ_CP019325
• Organism: Achromobacter insolitus strain DSM 23807

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BUW96_RS26980
• Protein ID: WP_076817034.1
• Coordinates: 5856086..5857438 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6S7FIA7
• Locus tag: BUW96_RS26975
• Protein ID: WP_042795666.1
• Coordinates: 5855824..5856081 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUW96_RS26960	5851475..5852505	+	1031	Protein_5337	sugar ABC transporter	-
BUW96_RS26965	5852498..5854402	+	1905	WP_076817032.1	Vi polysaccharide transport protein VexE	-
BUW96_RS26970	5854415..5855635	+	1221	WP_042795667.1	polysaccharide lyase family 1 protein	-
BUW96_RS26975	5855824..5856081	+	258	WP_042795666.1	helix-turn-helix domain-containing protein	Antitoxin
BUW96_RS26980	5856086..5857438	+	1353	WP_076817034.1	type II toxin-antitoxin system HipA family toxin	Toxin
BUW96_RS26985	5857469..5858236	-	768	WP_042795664.1	helix-turn-helix transcriptional regulator	-
BUW96_RS26990	5858329..5859450	+	1122	WP_076818077.1	MFS transporter	-
BUW96_RS26995	5859447..5860118	-	672	WP_063639549.1	Crp/Fnr family transcriptional regulator	-
BUW96_RS27000	5860345..5861559	+	1215	WP_088607648.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (159-377)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49403.60 Da
• Isoelectric Point: 7.7800

>T72250 WP_076817034.1 NZ_CP019325:5856086-5857438 [Achromobacter insolitus]
MGRRSHTQVLGLWMNGAYVGEWSNRPHAGEVLQYDKDWVASAPGRPLSLSLPFTPGNGPHRGDVVRAYFENLLPDSKEIR
ERVARRFRAGSTDAFALLAEIGRDCAGALQILPAGTAPADVASVQASPLNDAQVAQLLRSVLAPAPLGGLAIAEDDFRIS
IAGAQEKTALLRIDGQWCLPQGATPTTHILKLPLGLVGNMKFDLSESVENEWLCSEILAAYGMPVARAEVLSFEDMKVLA
VERFDRRWWDGEDGKRSLVRLPQEDMCQATGTPPHLKYEADGGPGIDRIMGLLETSRRSEHDRRTFFQAQVLFWMLCATD
GHAKNFSLFLRPGGAYELTPLYDVLSAYPILGEGPGKLSPVRAKMAMAVRSKNPHWRMRDIQRRHWLTVGARHGVVTPDG
KGVQFVVDDLIARTPEVVRAVRARLPQGFPRVLADSILDGLQAAADRLAA

Nucleotide

• Length: 1353 bp

>T72250 NZ_CP019325:5856086-5857438 [Achromobacter insolitus]
ATGGGGCGACGTTCACACACGCAGGTCCTGGGCCTATGGATGAATGGCGCCTATGTCGGAGAGTGGAGCAATCGACCCCA
CGCCGGCGAGGTCCTGCAGTACGACAAGGACTGGGTCGCCTCCGCGCCGGGCAGGCCATTGTCGCTGTCCCTGCCCTTCA
CGCCGGGCAATGGCCCGCATCGTGGCGACGTCGTCCGCGCATACTTCGAAAATCTGCTGCCTGACAGCAAGGAAATCCGC
GAACGGGTTGCGCGCCGGTTCCGGGCAGGCTCGACCGACGCGTTCGCCTTGCTTGCCGAGATCGGTCGCGATTGCGCGGG
CGCCTTGCAGATCCTGCCTGCGGGAACAGCGCCTGCAGACGTGGCGTCGGTGCAAGCCTCGCCCTTGAACGATGCCCAGG
TTGCGCAGCTGTTGCGCAGCGTTCTGGCGCCGGCGCCTCTCGGCGGCCTGGCCATCGCTGAGGATGATTTCCGGATTTCC
ATCGCAGGTGCGCAAGAGAAGACCGCCTTGCTGCGGATAGACGGCCAATGGTGCTTGCCCCAAGGCGCCACGCCGACGAC
TCACATCCTGAAGTTGCCATTGGGGCTGGTCGGCAATATGAAGTTCGACCTGAGCGAATCGGTGGAAAACGAATGGCTCT
GCTCGGAGATCTTGGCTGCCTACGGCATGCCCGTGGCGCGCGCGGAAGTACTGAGCTTCGAAGACATGAAGGTGCTCGCC
GTCGAACGTTTCGACCGCCGCTGGTGGGACGGCGAAGACGGAAAGCGCAGTCTAGTCCGCCTGCCCCAGGAAGACATGTG
CCAGGCCACCGGCACCCCGCCACACCTGAAGTACGAGGCGGACGGCGGACCCGGCATCGACCGCATCATGGGTCTGCTCG
AAACCTCGCGTCGCTCAGAGCACGACCGGCGAACGTTCTTCCAGGCGCAGGTTCTGTTCTGGATGCTGTGCGCGACGGAC
GGCCACGCGAAGAACTTCAGCCTGTTCCTGCGGCCGGGCGGGGCCTACGAACTTACACCGCTATACGACGTGCTGTCCGC
CTATCCGATCCTGGGCGAGGGACCAGGCAAGCTGTCGCCCGTTCGAGCGAAGATGGCGATGGCCGTGCGTTCGAAGAACC
CGCATTGGCGCATGCGCGATATCCAGCGGCGCCACTGGTTGACGGTGGGCGCGCGACATGGCGTCGTGACGCCGGACGGC
AAGGGAGTCCAATTCGTCGTTGACGATTTGATCGCGCGTACGCCAGAGGTCGTGCGTGCGGTCCGCGCCAGATTGCCGCA
AGGATTTCCGCGGGTGCTGGCGGACAGCATCCTGGACGGCTTGCAGGCCGCGGCGGACAGGCTCGCCGCGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9220.58 Da
• Isoelectric Point: 8.9814

>AT72250 WP_042795666.1 NZ_CP019325:5855824-5856081 [Achromobacter insolitus]
MDFTVRTATQLPALLQGFRKEAGLTQAATALRLGVTQQTLSALERNADKVSADRLLQLLSILGVEMVLRRQGAQPGPETT
SEPSW

Nucleotide

• Length: 258 bp

>AT72250 NZ_CP019325:5855824-5856081 [Achromobacter insolitus]
ATGGATTTCACCGTACGCACCGCGACACAATTGCCAGCCCTGCTCCAGGGGTTCCGCAAAGAGGCCGGCTTGACCCAGGC
GGCCACGGCCTTGCGCCTTGGGGTCACACAACAGACCTTGTCCGCCCTGGAGCGCAATGCCGACAAGGTCAGCGCAGACC
GGCTGCTGCAGCTGTTGAGCATCCTGGGAGTCGAAATGGTCTTGCGCCGGCAAGGGGCGCAGCCGGGACCCGAAACAACA
TCCGAACCCAGCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6S7FIA7