Detailed information of TA system TA072237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2035009..2035475
• Replicon: chromosome
• Accession: NZ_CP019306
• Organism: Fusobacterium necrophorum subsp. funduliforme strain F1260

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BWX37_RS10030
• Protein ID: WP_005961143.1
• Coordinates: 2035236..2035475 (+)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W0H3
• Locus tag: BWX37_RS10025
• Protein ID: WP_005955951.1
• Coordinates: 2035009..2035239 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWX37_RS10000	2030251..2030562	-	312	WP_005955931.1	50S ribosomal protein L21	-
BWX37_RS10005	2030687..2032354	-	1668	WP_005961152.1	tetratricopeptide repeat protein	-
BWX37_RS10010	2032397..2032873	-	477	WP_005955939.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
BWX37_RS10015	2032889..2033863	-	975	WP_005961153.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
BWX37_RS10020	2033989..2034507	-	519	WP_005961157.1	cob(I)yrinic acid a,c-diamide adenosyltransferase	-
BWX37_RS10025	2035009..2035239	+	231	WP_005955951.1	hypothetical protein	Antitoxin
BWX37_RS10030	2035236..2035475	+	240	WP_005961143.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWX37_RS10035	2035826..2036098	-	273	WP_009006013.1	hypothetical protein	-
BWX37_RS10040	2036098..2036490	-	393	WP_005960422.1	hypothetical protein	-
BWX37_RS10045	2036615..2037193	-	579	WP_062623699.1	N-acetylmuramoyl-L-alanine amidase	-
BWX37_RS10050	2037190..2037876	-	687	WP_062623700.1	DUF4376 domain-containing protein	-
BWX37_RS10055	2037880..2038362	-	483	WP_062623701.1	hypothetical protein	-
BWX37_RS10060	2038349..2039893	-	1545	WP_123383018.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2032889..2089941	57052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-78)

Antitoxin

• Protein of unknown function DUF6290 (1-72)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9566.32 Da
• Isoelectric Point: 10.2840

>T72237 WP_005961143.1 NZ_CP019306:2035236-2035475 [Fusobacterium necrophorum subsp. funduliforme]
MKYRIEFSQQGKKELKKLDAFTQKIIMKWIYKNLIDTENPRIHGKELKGNLKCFWRYRVGNYRLLADIQDETVTILLMT

Nucleotide

• Length: 240 bp

>T72237 NZ_CP019306:2035236-2035475 [Fusobacterium necrophorum subsp. funduliforme]
ATGAAATACCGAATAGAATTTTCACAACAAGGAAAAAAGGAATTGAAAAAATTAGATGCCTTTACTCAAAAAATTATTAT
GAAGTGGATCTATAAAAATCTAATTGATACGGAAAATCCAAGAATACATGGAAAAGAACTAAAGGGAAACTTAAAGTGTT
TTTGGCGTTATCGTGTCGGAAATTATAGGTTATTAGCCGATATTCAAGACGAAACGGTAACCATTTTATTAATGACATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9012.25 Da
• Isoelectric Point: 4.3363

>AT72237 WP_005955951.1 NZ_CP019306:2035009-2035239 [Fusobacterium necrophorum subsp. funduliforme]
MSIISLRLNEKEEKLLREFSAFEGLGISSYIKKIIYERLEDEYDIKCFDKAYQEYLESGKKSYSFDEVLNELGIEL

Nucleotide

• Length: 231 bp

>AT72237 NZ_CP019306:2035009-2035239 [Fusobacterium necrophorum subsp. funduliforme]
ATGTCTATTATTTCTTTACGACTAAATGAAAAAGAAGAAAAATTATTAAGAGAATTCTCAGCATTTGAGGGCTTGGGAAT
CTCCTCATATATCAAAAAAATTATCTATGAAAGATTAGAGGATGAGTATGATATTAAATGTTTTGATAAAGCATATCAGG
AATATCTGGAAAGTGGAAAAAAATCATATTCTTTTGATGAAGTTTTAAATGAATTGGGAATTGAGCTATGA

Structures

Antitoxin

• AlphaFold DB: J5W0H3