Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2598780..2599431 Replicon chromosome
Accession NZ_CP019305
Organism Lacticaseibacillus rhamnosus strain SCT-10-10-60

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag BWQ99_RS13040 Protein ID WP_005686631.1
Coordinates 2598780..2599163 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag BWQ99_RS13045 Protein ID WP_005686632.1
Coordinates 2599183..2599431 (-) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWQ99_RS13025 2597739..2598266 - 528 WP_005691221.1 QueT transporter family protein -
BWQ99_RS13040 2598780..2599163 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BWQ99_RS13045 2599183..2599431 - 249 WP_005686632.1 antitoxin Antitoxin
BWQ99_RS13050 2599543..2600682 - 1140 WP_005691215.1 alanine racemase -
BWQ99_RS13055 2600669..2601043 - 375 WP_005686634.1 holo-ACP synthase -
BWQ99_RS13060 2601212..2602720 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
BWQ99_RS13065 2602994..2604382 - 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-115)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T72235 WP_005686631.1 NZ_CP019305:c2599163-2598780 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T72235 NZ_CP019305:c2599163-2598780 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT72235 WP_005686632.1 NZ_CP019305:c2599431-2599183 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT72235 NZ_CP019305:c2599431-2599183 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB K8Q4Y0


Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References