Detailed information of TA system TA072221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 351096..351655
• Replicon: chromosome
• Accession: NZ_CP019291
• Organism: Vibrio vulnificus strain FORC_053

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FORC53_RS17690
• Protein ID: WP_047111817.1
• Coordinates: 351377..351655 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3Q0L0M2
• Locus tag: FORC53_RS17685
• Protein ID: WP_011082348.1
• Coordinates: 351096..351380 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC53_RS17655	346241..347434	+	1194	WP_011082341.1	glycine C-acetyltransferase	-
FORC53_RS17660	347471..348496	+	1026	WP_197672410.1	L-threonine 3-dehydrogenase	-
FORC53_RS17665	348576..349076	-	501	WP_065090958.1	hypothetical protein	-
FORC53_RS17670	349154..349417	-	264	WP_011082346.1	hypothetical protein	-
FORC53_RS17675	349602..350135	-	534	WP_118894208.1	hypothetical protein	-
FORC53_RS17680	350270..350878	-	609	WP_011151714.1	hypothetical protein	-
FORC53_RS17685	351096..351380	+	285	WP_011082348.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FORC53_RS17690	351377..351655	+	279	WP_047111817.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
FORC53_RS17695	351684..351750	-	67	Protein_281	DUF3265 domain-containing protein	-
FORC53_RS17700	351810..353594	-	1785	WP_065090960.1	NACHT domain-containing protein	-
FORC53_RS17705	353969..355678	-	1710	WP_118894209.1	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	349154..362594	13440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-45)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10951.63 Da
• Isoelectric Point: 4.3430

>T72221 WP_047111817.1 NZ_CP019291:351377-351655 [Vibrio vulnificus]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDNLIETKVENLLEQPLMGVQRDSIRGRLLIIPEISMVISYWVEGDIIRVM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T72221 NZ_CP019291:351377-351655 [Vibrio vulnificus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAACAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATAGCA
TCCGCGGTCGATTACTCATTATTCCCGAGATTTCGATGGTTATCTCTTACTGGGTCGAGGGCGATATCATCCGAGTTATG
CGTGTCCTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11083.43 Da
• Isoelectric Point: 9.2167

>AT72221 WP_011082348.1 NZ_CP019291:351096-351380 [Vibrio vulnificus]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKFDSGKSVFIEHQTAKSR
MEERKARIRNRGKQ

Nucleotide

• Length: 285 bp

>AT72221 NZ_CP019291:351096-351380 [Vibrio vulnificus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCAGAGAGTCAAGGTCGCAC
TCTTAGTGACGCATGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTGTCTCACGATGCATGGCTAA
CTGAACAAGTAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCATTGAGCACCAAACTGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGGGGTAAGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0L0M2