Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3232650..3233448 Replicon chromosome
Accession NZ_CP019280
Organism Escherichia coli strain 13P484A

Toxin (Protein)


Gene name yeeV Uniprot ID U9XMP3
Locus tag BWI90_RS16950 Protein ID WP_000854735.1
Coordinates 3233071..3233448 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1EW42
Locus tag BWI90_RS16945 Protein ID WP_032153712.1
Coordinates 3232650..3233024 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI90_RS16905 3228383..3229267 + 885 WP_024166630.1 50S ribosome-binding GTPase -
BWI90_RS16910 3229386..3230063 + 678 WP_023155728.1 hypothetical protein -
BWI90_RS16915 3230093..3230302 + 210 WP_023155727.1 DUF905 family protein -
BWI90_RS16920 3230393..3231211 + 819 WP_001234693.1 DUF945 domain-containing protein -
BWI90_RS16925 3231303..3231788 + 486 WP_032153711.1 antirestriction protein -
BWI90_RS16935 3231804..3232280 + 477 WP_001186715.1 RadC family protein -
BWI90_RS16940 3232349..3232570 + 222 WP_000692345.1 DUF987 domain-containing protein -
BWI90_RS16945 3232650..3233024 + 375 WP_032153712.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
BWI90_RS16950 3233071..3233448 + 378 WP_000854735.1 TA system toxin CbtA family protein Toxin
BWI90_RS16955 3233445..3233936 + 492 WP_023155722.1 hypothetical protein -
BWI90_RS16960 3233948..3234145 + 198 WP_000839265.1 DUF957 domain-containing protein -
BWI90_RS16965 3234230..3235096 + 867 WP_032153714.1 DUF4942 domain-containing protein -
BWI90_RS16970 3235168..3235431 + 264 WP_025492088.1 type II toxin-antitoxin system ParD family antitoxin -
BWI90_RS16975 3235428..3235754 + 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
BWI90_RS16980 3236218..3237480 + 1263 WP_148119787.1 integrase arm-type DNA-binding domain-containing protein -
BWI90_RS16990 3237666..3238355 - 690 Protein_3077 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3237844..3238161 317


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14061.07 Da        Isoelectric Point: 8.2904

>T72204 WP_000854735.1 NZ_CP019280:3233071-3233448 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T72204 NZ_CP019280:3233071-3233448 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13697.51 Da        Isoelectric Point: 6.6240

>AT72204 WP_032153712.1 NZ_CP019280:3232650-3233024 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT72204 NZ_CP019280:3232650-3233024 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XMP3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1EW42

References