Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1700710..1701236 Replicon chromosome
Accession NZ_CP019280
Organism Escherichia coli strain 13P484A

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BWI90_RS08975 Protein ID WP_000323025.1
Coordinates 1700710..1700997 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BWI90_RS08980 Protein ID WP_000534858.1
Coordinates 1700997..1701236 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI90_RS08940 1696392..1697351 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
BWI90_RS08945 1697544..1698068 + 525 WP_000780584.1 lipocalin family protein -
BWI90_RS08950 1698224..1698601 - 378 WP_001204787.1 antitermination protein -
BWI90_RS08955 1698619..1699668 - 1050 WP_073527870.1 DUF968 domain-containing protein -
BWI90_RS08960 1699670..1699948 - 279 WP_012304870.1 hypothetical protein -
BWI90_RS08965 1700015..1700266 - 252 WP_000980994.1 hypothetical protein -
BWI90_RS08970 1700483..1700638 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BWI90_RS08975 1700710..1700997 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BWI90_RS08980 1700997..1701236 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BWI90_RS08985 1701261..1701566 + 306 WP_001326990.1 hypothetical protein -
BWI90_RS08990 1701769..1702101 + 333 WP_073527871.1 FlxA-like family protein -
BWI90_RS09000 1702538..1703879 - 1342 Protein_1632 ISNCY family transposase -
BWI90_RS27805 1704648..1704935 - 288 Protein_1633 hypothetical protein -
BWI90_RS09015 1705546..1705902 - 357 WP_001374839.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1655749..1714378 58629


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T72191 WP_000323025.1 NZ_CP019280:c1700997-1700710 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T72191 NZ_CP019280:c1700997-1700710 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT72191 WP_000534858.1 NZ_CP019280:c1701236-1700997 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT72191 NZ_CP019280:c1701236-1700997 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References