Detailed information of TA system TA072184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 917804..918509
• Replicon: chromosome
• Accession: NZ_CP019280
• Organism: Escherichia coli strain 13P484A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: BWI90_RS04805
• Protein ID: WP_000539521.1
• Coordinates: 918123..918509 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BWI90_RS04800
• Protein ID: WP_001280945.1
• Coordinates: 917804..918133 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI90_RS04785	912961..913872	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
BWI90_RS04790	914050..916398	+	2349	WP_001338403.1	EAL domain-containing protein	-
BWI90_RS04795	916406..917734	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
BWI90_RS04800	917804..918133	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
BWI90_RS04805	918123..918509	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWI90_RS04810	918735..920060	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
BWI90_RS04815	920273..920656	+	384	WP_000497137.1	biofilm formation regulator BssR	-
BWI90_RS04820	920767..921882	+	1116	Protein_865	aldose sugar dehydrogenase YliI	-
BWI90_RS04830	921879..922505	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T72184 WP_000539521.1 NZ_CP019280:c918509-918123 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T72184 NZ_CP019280:c918509-918123 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT72184 WP_001280945.1 NZ_CP019280:c918133-917804 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT72184 NZ_CP019280:c918133-917804 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATAA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6