Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 493951..494569 Replicon chromosome
Accession NZ_CP019280
Organism Escherichia coli strain 13P484A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BWI90_RS02495 Protein ID WP_001291435.1
Coordinates 493951..494169 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BWI90_RS02500 Protein ID WP_000344800.1
Coordinates 494195..494569 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI90_RS02450 489066..489377 - 312 WP_000409911.1 MGMT family protein -
BWI90_RS02460 489756..490109 + 354 Protein_453 DUF1428 family protein -
BWI90_RS02465 490151..491317 - 1167 Protein_454 cyclic-guanylate-specific phosphodiesterase PdeB -
BWI90_RS02475 491373..492070 + 698 Protein_455 IS1-like element IS1A family transposase -
BWI90_RS02480 492086..492478 - 393 Protein_456 cyclic-guanylate-specific phosphodiesterase PdeB -
BWI90_RS02485 492642..493112 - 471 WP_000136192.1 YlaC family protein -
BWI90_RS02490 493228..493779 - 552 WP_148119680.1 maltose O-acetyltransferase -
BWI90_RS02495 493951..494169 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BWI90_RS02500 494195..494569 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BWI90_RS02505 495115..498264 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BWI90_RS02510 498287..499480 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T72183 WP_001291435.1 NZ_CP019280:c494169-493951 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T72183 NZ_CP019280:c494169-493951 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT72183 WP_000344800.1 NZ_CP019280:c494569-494195 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT72183 NZ_CP019280:c494569-494195 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References