Detailed information of TA system TA072180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 61879..62372
• Replicon: plasmid p13P477T-4
• Accession: NZ_CP019277
• Organism: Escherichia coli strain 13P477T

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: BWI89_RS27785
• Protein ID: WP_001230709.1
• Coordinates: 61879..62133 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: BWI89_RS27790
• Protein ID: WP_000151456.1
• Coordinates: 62130..62372 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI89_RS27745	57451..57612	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
BWI89_RS27750	57762..58358	+	597	WP_000125164.1	hypothetical protein	-
BWI89_RS29750	58376..58660	-	285	WP_001162371.1	hypothetical protein	-
BWI89_RS27760	58734..59399	-	666	WP_000532148.1	P-loop NTPase	-
BWI89_RS27770	59988..60317	+	330	WP_000855119.1	hypothetical protein	-
BWI89_RS27775	60721..61236	+	516	WP_001025393.1	J domain-containing protein	-
BWI89_RS27780	61340..61882	+	543	WP_001083904.1	hypothetical protein	-
BWI89_RS27785	61879..62133	+	255	WP_001230709.1	hypothetical protein	Toxin
BWI89_RS27790	62130..62372	+	243	WP_000151456.1	hypothetical protein	Antitoxin
BWI89_RS27795	62593..62811	+	219	WP_154101533.1	hypothetical protein	-
BWI89_RS27800	62943..63236	+	294	WP_000673428.1	hypothetical protein	-
BWI89_RS27805	63649..63843	-	195	WP_065358362.1	hypothetical protein	-
BWI89_RS27810	64401..64583	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
BWI89_RS27815	64608..65045	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
BWI89_RS27820	65176..65496	-	321	WP_048238069.1	hypothetical protein	-
BWI89_RS27825	65500..65787	-	288	WP_000212745.1	hypothetical protein	-
BWI89_RS29450	65789..65977	-	189	WP_000797279.1	hypothetical protein	-
BWI89_RS29755	65974..66150	-	177	WP_001261533.1	hypothetical protein	-
BWI89_RS27830	66150..66629	-	480	WP_065358359.1	ead/Ea22-like family protein	-
BWI89_RS27835	66626..66925	-	300	WP_077248630.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..105415	105415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T72180 WP_001230709.1 NZ_CP019277:61879-62133 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T72180 NZ_CP019277:61879-62133 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATCAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT72180 WP_000151456.1 NZ_CP019277:62130-62372 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT72180 NZ_CP019277:62130-62372 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5