Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4577748..4578549 | Replicon | chromosome |
| Accession | NZ_CP019273 | ||
| Organism | Escherichia coli strain 13P477T | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D8EBK0 |
| Locus tag | BWI89_RS23935 | Protein ID | WP_001094436.1 |
| Coordinates | 4578172..4578549 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | B7MN76 |
| Locus tag | BWI89_RS23930 | Protein ID | WP_015953067.1 |
| Coordinates | 4577748..4578125 (+) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BWI89_RS23880 | 4572840..4573544 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| BWI89_RS23885 | 4573609..4573788 | - | 180 | Protein_4354 | winged helix-turn-helix domain-containing protein | - |
| BWI89_RS23890 | 4574095..4574799 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| BWI89_RS23895 | 4574857..4575165 | + | 309 | WP_072132004.1 | hypothetical protein | - |
| BWI89_RS23900 | 4575171..4575404 | + | 234 | WP_001278287.1 | DUF905 family protein | - |
| BWI89_RS23905 | 4575494..4576312 | + | 819 | WP_001175142.1 | DUF945 domain-containing protein | - |
| BWI89_RS23910 | 4576403..4576888 | + | 486 | WP_000860054.1 | antirestriction protein | - |
| BWI89_RS23920 | 4576903..4577379 | + | 477 | WP_001186756.1 | RadC family protein | - |
| BWI89_RS23925 | 4577448..4577669 | + | 222 | WP_000692312.1 | DUF987 domain-containing protein | - |
| BWI89_RS23930 | 4577748..4578125 | + | 378 | WP_015953067.1 | type IV toxin-antitoxin system toxin CbtA | Antitoxin |
| BWI89_RS23935 | 4578172..4578549 | + | 378 | WP_001094436.1 | TA system toxin CbtA family protein | Toxin |
| BWI89_RS23940 | 4578546..4579034 | + | 489 | WP_000761714.1 | hypothetical protein | - |
| BWI89_RS23945 | 4579046..4579243 | + | 198 | WP_000839254.1 | DUF957 domain-containing protein | - |
| BWI89_RS23950 | 4579328..4580173 | + | 846 | WP_072692382.1 | DUF4942 domain-containing protein | - |
| BWI89_RS23955 | 4580244..4581779 | + | 1536 | WP_000492897.1 | EAL domain-containing protein | - |
| BWI89_RS23960 | 4582114..4582650 | - | 537 | WP_000942807.1 | GspM family type II secretion system protein YghD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 | rfaE / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC | 4429227..4598400 | 169173 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13958.84 Da Isoelectric Point: 6.8603
>T72170 WP_001094436.1 NZ_CP019273:4578172-4578549 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ
Download Length: 378 bp
>T72170 NZ_CP019273:4578172-4578549 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13766.60 Da Isoelectric Point: 6.2021
>AT72170 WP_015953067.1 NZ_CP019273:4577748-4578125 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
Download Length: 378 bp
>AT72170 NZ_CP019273:4577748-4578125 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E2L1N0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Z3CIS0 |