Detailed information of TA system TA072166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4001814..4002409
• Replicon: chromosome
• Accession: NZ_CP019273
• Organism: Escherichia coli strain 13P477T

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: BWI89_RS20705
• Protein ID: WP_000155159.1
• Coordinates: 4002032..4002409 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: BWI89_RS20700
• Protein ID: WP_000557315.1
• Coordinates: 4001814..4002035 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI89_RS20675	3996890..3997999	+	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
BWI89_RS20680	3998047..3998973	+	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
BWI89_RS20685	3998970..4000247	+	1278	WP_000803825.1	branched chain amino acid ABC transporter permease LivM	-
BWI89_RS20690	4000244..4001011	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
BWI89_RS20695	4001013..4001726	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
BWI89_RS20700	4001814..4002035	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BWI89_RS20705	4002032..4002409	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BWI89_RS20710	4002618..4003934	+	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
BWI89_RS20715	4004110..4004997	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
BWI89_RS20720	4004994..4005839	+	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
BWI89_RS20725	4005841..4006911	+	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3991702..4007651	15949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T72166 WP_000155159.1 NZ_CP019273:4002032-4002409 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T72166 NZ_CP019273:4002032-4002409 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT72166 WP_000557315.1 NZ_CP019273:4001814-4002035 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT72166 NZ_CP019273:4001814-4002035 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32