Detailed information of TA system TA072153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1357769..1358405
• Replicon: chromosome
• Accession: NZ_CP019273
• Organism: Escherichia coli strain 13P477T

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: BWI89_RS07010
• Protein ID: WP_000813796.1
• Coordinates: 1358229..1358405 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BWI89_RS07005
• Protein ID: WP_076838470.1
• Coordinates: 1357769..1358185 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI89_RS06985	1352920..1353861	-	942	WP_001251307.1	ABC transporter permease	-
BWI89_RS06990	1353862..1354875	-	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
BWI89_RS06995	1354893..1356038	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
BWI89_RS07000	1356283..1357690	-	1408	Protein_1279	PLP-dependent aminotransferase family protein	-
BWI89_RS07005	1357769..1358185	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BWI89_RS07010	1358229..1358405	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BWI89_RS07015	1358627..1358857	+	231	WP_000494243.1	YncJ family protein	-
BWI89_RS07020	1358949..1360910	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BWI89_RS07025	1360983..1361519	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
BWI89_RS07030	1361572..1362783	+	1212	WP_071593090.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T72153 WP_000813796.1 NZ_CP019273:c1358405-1358229 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T72153 NZ_CP019273:c1358405-1358229 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT72153 WP_076838470.1 NZ_CP019273:c1358185-1357769 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT72153 NZ_CP019273:c1358185-1357769 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3