Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 33600..34122 Replicon plasmid p13P460A-1
Accession NZ_CP019272
Organism Escherichia coli strain 13P460A

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag BWI88_RS24665 Protein ID WP_000220561.1
Coordinates 33600..33881 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag BWI88_RS24670 Protein ID WP_000121743.1
Coordinates 33871..34122 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI88_RS24635 29076..29312 - 237 Protein_33 hypothetical protein -
BWI88_RS24640 29333..30127 - 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
BWI88_RS24645 30156..31586 - 1431 Protein_35 IS4-like element IS50R family transposase -
BWI88_RS24650 31706..31894 + 189 WP_000957857.1 hypothetical protein -
BWI88_RS24655 31904..33103 + 1200 WP_000948429.1 IS91 family transposase -
BWI88_RS26665 33269..33361 + 93 Protein_38 IS6 family transposase -
BWI88_RS24665 33600..33881 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BWI88_RS24670 33871..34122 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
BWI88_RS24680 35357..36196 + 840 WP_024129959.1 replication initiation protein -
BWI88_RS24685 36217..37059 + 843 WP_000435064.1 replication initiation protein -
BWI88_RS24690 37063..37509 + 447 WP_001074384.1 hypothetical protein -
BWI88_RS24695 37577..37999 + 423 WP_179292036.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / oqxA / oqxB / aph(3')-IIa / fosA3 / blaTEM-1B / blaCTX-M-55 - 1..129452 129452
- flank IS/Tn - - 38485..39684 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T72133 WP_000220561.1 NZ_CP019272:c33881-33600 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T72133 NZ_CP019272:c33881-33600 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT72133 WP_000121743.1 NZ_CP019272:c34122-33871 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT72133 NZ_CP019272:c34122-33871 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References