Detailed information of TA system TA072131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4256228..4256866
• Replicon: chromosome
• Accession: NZ_CP019271
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: BWI88_RS21800
• Protein ID: WP_000813794.1
• Coordinates: 4256690..4256866 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BWI88_RS21795
• Protein ID: WP_001270286.1
• Coordinates: 4256228..4256644 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS21775	4251380..4252321	-	942	WP_001251319.1	ABC transporter permease	-
BWI88_RS21780	4252322..4253335	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
BWI88_RS21785	4253353..4254498	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
BWI88_RS21790	4254743..4256149	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
BWI88_RS21795	4256228..4256644	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BWI88_RS21800	4256690..4256866	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BWI88_RS21805	4257088..4257318	+	231	WP_000494244.1	YncJ family protein	-
BWI88_RS21810	4257410..4259371	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BWI88_RS21815	4259444..4259980	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
BWI88_RS21820	4260033..4261247	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4261287..4262552	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T72131 WP_000813794.1 NZ_CP019271:c4256866-4256690 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T72131 NZ_CP019271:c4256866-4256690 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT72131 WP_001270286.1 NZ_CP019271:c4256644-4256228 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT72131 NZ_CP019271:c4256644-4256228 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB