Detailed information of TA system TA072121

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3630276..3631111
• Replicon: chromosome
• Accession: NZ_CP019271
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2T3T4L0
• Locus tag: BWI88_RS18415
• Protein ID: WP_001556084.1
• Coordinates: 3630276..3630653 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A403D757
• Locus tag: BWI88_RS18420
• Protein ID: WP_016230789.1
• Coordinates: 3630743..3631111 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS18380	3625540..3625722	-	183	WP_024174802.1	ethanolamine utilization protein	-
BWI88_RS26780	3626176..3626565	-	390	WP_102804527.1	transposase	-
BWI88_RS26785	3627363..3627476	-	114	WP_032140621.1	DUF957 domain-containing protein	-
BWI88_RS18400	3627673..3629214	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
BWI88_RS18405	3629229..3629975	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
BWI88_RS18410	3630076..3630279	-	204	WP_000761705.1	hypothetical protein	-
BWI88_RS18415	3630276..3630653	-	378	WP_001556084.1	TA system toxin CbtA family protein	Toxin
BWI88_RS18420	3630743..3631111	-	369	WP_016230789.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BWI88_RS18425	3631274..3631495	-	222	WP_000692345.1	DUF987 domain-containing protein	-
BWI88_RS18430	3631558..3632034	-	477	WP_001186775.1	RadC family protein	-
BWI88_RS18435	3632050..3632523	-	474	WP_001765426.1	antirestriction protein	-
BWI88_RS18445	3632865..3633683	-	819	WP_020239663.1	DUF945 domain-containing protein	-
BWI88_RS18455	3633838..3633996	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3627673..3682463	54790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14232.27 Da
• Isoelectric Point: 7.8276

>T72121 WP_001556084.1 NZ_CP019271:c3630653-3630276 [Escherichia coli]
MKTLPDTHVRELSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T72121 NZ_CP019271:c3630653-3630276 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGTTATCGCGCTGCCCATCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13614.45 Da
• Isoelectric Point: 7.0264

>AT72121 WP_016230789.1 NZ_CP019271:c3631111-3630743 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT72121 NZ_CP019271:c3631111-3630743 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2T3T4L0

Antitoxin

• AlphaFold DB: A0A403D757