Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2710021..2710675 Replicon chromosome
Accession NZ_CP019271
Organism Escherichia coli strain 13P460A

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag BWI88_RS13835 Protein ID WP_000244772.1
Coordinates 2710268..2710675 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BWI88_RS13830 Protein ID WP_000354046.1
Coordinates 2710021..2710287 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI88_RS13805 2705309..2706052 + 744 WP_000951964.1 SDR family oxidoreductase -
BWI88_RS13810 2706109..2707542 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
BWI88_RS13815 2707587..2707898 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
BWI88_RS13820 2708062..2708721 + 660 WP_000250274.1 hemolysin III family protein -
BWI88_RS13825 2708798..2709778 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BWI88_RS13830 2710021..2710287 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BWI88_RS13835 2710268..2710675 + 408 WP_000244772.1 protein YgfX Toxin
BWI88_RS13840 2710715..2711236 - 522 WP_001055874.1 flavodoxin FldB -
BWI88_RS13845 2711348..2712244 + 897 WP_148125462.1 site-specific tyrosine recombinase XerD -
BWI88_RS13850 2712269..2712979 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWI88_RS13855 2712985..2714718 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T72117 WP_000244772.1 NZ_CP019271:2710268-2710675 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T72117 NZ_CP019271:2710268-2710675 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT72117 WP_000354046.1 NZ_CP019271:2710021-2710287 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT72117 NZ_CP019271:2710021-2710287 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References