Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61542..62185 Replicon plasmid p13C1079T-2
Accession NZ_CP019269
Organism Escherichia coli strain 13C1079T

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag BWI87_RS25700 Protein ID WP_001044768.1
Coordinates 61542..61958 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag BWI87_RS25705 Protein ID WP_001261287.1
Coordinates 61955..62185 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI87_RS25685 58041..58631 - 591 WP_000194575.1 hypothetical protein -
BWI87_RS25690 58631..58888 - 258 WP_000343085.1 hypothetical protein -
BWI87_RS25695 59242..61380 + 2139 WP_000350638.1 AAA family ATPase -
BWI87_RS25700 61542..61958 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
BWI87_RS25705 61955..62185 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BWI87_RS25715 62481..62771 + 291 WP_000111771.1 hypothetical protein -
BWI87_RS25720 62945..63133 + 189 WP_000957857.1 hypothetical protein -
BWI87_RS25725 63143..64350 + 1208 Protein_80 IS91 family transposase -
BWI87_RS26065 64512..64603 + 92 Protein_81 IS6 family transposase -
BWI87_RS25735 64669..64997 + 329 Protein_82 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-55 / blaTEM-1B / fosA3 - 1..65009 65009
- flank IS/Tn - - 63143..64021 878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T72105 WP_001044768.1 NZ_CP019269:c61958-61542 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T72105 NZ_CP019269:c61958-61542 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT72105 WP_001261287.1 NZ_CP019269:c62185-61955 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT72105 NZ_CP019269:c62185-61955 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References