Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12353..12996 Replicon plasmid p13C1079T-2
Accession NZ_CP019269
Organism Escherichia coli strain 13C1079T

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag BWI87_RS25315 Protein ID WP_001044768.1
Coordinates 12353..12769 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag BWI87_RS25320 Protein ID WP_001261287.1
Coordinates 12766..12996 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI87_RS25300 8852..9442 - 591 WP_000194575.1 hypothetical protein -
BWI87_RS25305 9442..9699 - 258 WP_000343085.1 hypothetical protein -
BWI87_RS25310 10053..12191 + 2139 WP_000350638.1 AAA family ATPase -
BWI87_RS25315 12353..12769 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
BWI87_RS25320 12766..12996 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BWI87_RS25330 13292..13582 + 291 WP_000111771.1 hypothetical protein -
BWI87_RS25335 13756..13944 + 189 WP_000957857.1 hypothetical protein -
BWI87_RS25340 13954..15153 + 1200 WP_000948429.1 IS91 family transposase -
BWI87_RS26045 15319..15411 + 93 Protein_16 IS6 family transposase -
BWI87_RS25350 15477..16022 + 546 Protein_17 Tn3 family transposase -
BWI87_RS25355 16058..16473 - 416 Protein_18 IS1 family transposase -
BWI87_RS25370 16722..17123 - 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
BWI87_RS26050 17056..17313 - 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-55 / blaTEM-1B / fosA3 - 1..65009 65009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T72100 WP_001044768.1 NZ_CP019269:c12769-12353 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T72100 NZ_CP019269:c12769-12353 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT72100 WP_001261287.1 NZ_CP019269:c12996-12766 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT72100 NZ_CP019269:c12996-12766 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References