Detailed information of TA system TA072091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3965901..3966526
• Replicon: chromosome
• Accession: NZ_CP019267
• Organism: Escherichia coli strain 13C1079T

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWI87_RS20365
• Protein ID: WP_000911330.1
• Coordinates: 3965901..3966299 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BWI87_RS20370
• Protein ID: WP_000450524.1
• Coordinates: 3966299..3966526 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI87_RS20345	3961779..3961979	+	201	WP_000383836.1	YpfN family protein	-
BWI87_RS20350	3962089..3962787	-	699	WP_000679823.1	esterase	-
BWI87_RS20355	3962861..3964876	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
BWI87_RS20360	3964891..3965754	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
BWI87_RS20365	3965901..3966299	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BWI87_RS20370	3966299..3966526	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BWI87_RS20375	3966680..3967393	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BWI87_RS20380	3967606..3968640	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
BWI87_RS20385	3968657..3969535	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BWI87_RS20390	3969681..3970253	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
BWI87_RS20395	3970253..3970723	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T72091 WP_000911330.1 NZ_CP019267:c3966299-3965901 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T72091 NZ_CP019267:c3966299-3965901 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT72091 WP_000450524.1 NZ_CP019267:c3966526-3966299 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT72091 NZ_CP019267:c3966526-3966299 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33