Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2848329..2848967 Replicon chromosome
Accession NZ_CP019267
Organism Escherichia coli strain 13C1079T

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BWI87_RS14635 Protein ID WP_000813794.1
Coordinates 2848329..2848505 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BWI87_RS14640 Protein ID WP_001270286.1
Coordinates 2848551..2848967 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI87_RS14615 2843948..2845162 - 1215 WP_001349372.1 BenE family transporter YdcO -
BWI87_RS14620 2845215..2845751 + 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
BWI87_RS14625 2845824..2847785 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BWI87_RS14630 2847877..2848107 - 231 WP_000494244.1 YncJ family protein -
BWI87_RS14635 2848329..2848505 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BWI87_RS14640 2848551..2848967 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BWI87_RS14645 2849046..2850452 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
BWI87_RS14650 2850697..2851842 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
BWI87_RS14655 2851860..2852873 + 1014 WP_148116094.1 ABC transporter ATP-binding protein -
BWI87_RS14660 2852874..2853815 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2842643..2843908 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T72081 WP_000813794.1 NZ_CP019267:2848329-2848505 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T72081 NZ_CP019267:2848329-2848505 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT72081 WP_001270286.1 NZ_CP019267:2848551-2848967 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT72081 NZ_CP019267:2848551-2848967 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References