72063

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 37405..37898 Replicon plasmid p13C1065T-5
Accession NZ_CP019264
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag BWI86_RS27785 Protein ID WP_001230709.1
Coordinates 37644..37898 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag BWI86_RS27780 Protein ID WP_000151456.1
Coordinates 37405..37647 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS27745 32728..33039 - 312 WP_001310436.1 hypothetical protein -
BWI86_RS27750 33036..33452 - 417 WP_053882499.1 phage tail protein -
BWI86_RS27755 33462..33749 - 288 WP_000673430.1 hypothetical protein -
BWI86_RS27760 33865..34114 - 250 Protein_38 hypothetical protein -
BWI86_RS27765 34499..35761 + 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
BWI86_RS27770 36014..36889 + 876 WP_015387340.1 class A extended-spectrum beta-lactamase CTX-M-55 -
BWI86_RS27775 36936..37268 - 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
BWI86_RS27780 37405..37647 - 243 WP_000151456.1 hypothetical protein Antitoxin
BWI86_RS27785 37644..37898 - 255 WP_001230709.1 hypothetical protein Toxin
BWI86_RS27790 37895..38437 - 543 WP_001083904.1 hypothetical protein -
BWI86_RS27795 38541..39056 - 516 WP_001025393.1 J domain-containing protein -
BWI86_RS27805 39460..39789 - 330 WP_000855119.1 hypothetical protein -
BWI86_RS27815 40376..41041 + 666 WP_015387337.1 P-loop NTPase -
BWI86_RS29285 41115..41399 + 285 WP_001162371.1 hypothetical protein -
BWI86_RS27825 41417..42013 - 597 WP_000125165.1 hypothetical protein -
BWI86_RS27830 42162..42323 - 162 WP_001434039.1 type I toxin-antitoxin system Hok family toxin -
BWI86_RS29290 42517..42678 - 162 WP_001128311.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 / blaCTX-M-55 - 1..82265 82265
- flank IS/Tn blaCTX-M-55 - 34499..36889 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(12-128)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T72063 WP_001230709.1 NZ_CP019264:c37898-37644 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T72063 NZ_CP019264:c37898-37644 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT72063 WP_000151456.1 NZ_CP019264:c37647-37405 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT72063 NZ_CP019264:c37647-37405 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References