Detailed information of TA system TA072061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 45928..46529
• Replicon: plasmid p13C1065T-3
• Accession: NZ_CP019262
• Organism: Escherichia coli strain 13C1065T

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: BWI86_RS26740
• Protein ID: WP_109157903.1
• Coordinates: 46149..46529 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: BWI86_RS26735
• Protein ID: WP_001190712.1
• Coordinates: 45928..46149 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI86_RS26725	42948..44192	-	1245	WP_148116451.1	restriction endonuclease subunit S	-
BWI86_RS26730	44189..45745	-	1557	WP_001617892.1	type I restriction-modification system subunit M	-
BWI86_RS26735	45928..46149	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BWI86_RS26740	46149..46529	+	381	WP_109157903.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BWI86_RS26745	46534..46713	+	180	WP_001411052.1	hypothetical protein	-
BWI86_RS26750	46741..47784	+	1044	WP_148116453.1	DUF968 domain-containing protein	-
BWI86_RS26755	47873..48325	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
BWI86_RS26760	48408..49601	+	1194	WP_023154372.1	terminase A protein	-
BWI86_RS26765	49601..51085	+	1485	WP_148116454.1	terminase	-
BWI86_RS28860	51307..51426	+	120	WP_071526433.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / dfrA1 / ant(3'')-Ia / qacE / sul1 / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / oqxB / oqxA	-	1..149653	149653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.34 Da
• Isoelectric Point: 5.6343

>T72061 WP_109157903.1 NZ_CP019262:46149-46529 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGTATGEISVSTVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T72061 NZ_CP019262:46149-46529 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGACTGCGACTGG
CGAGATATCTGTATCTACTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT72061 WP_001190712.1 NZ_CP019262:45928-46149 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT72061 NZ_CP019262:45928-46149 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6