Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 27414..27949 Replicon plasmid p13C1065T-2
Accession NZ_CP019261
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag BWI86_RS25740 Protein ID WP_000222766.1
Coordinates 27414..27701 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag BWI86_RS25745 Protein ID WP_001132900.1
Coordinates 27698..27949 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS25705 22962..23822 - 861 WP_000557454.1 aminoglycoside N-acetyltransferase AAC(3)-IId -
BWI86_RS25715 23965..24459 + 495 Protein_28 IS4-like element ISVsa5 family transposase -
BWI86_RS25720 24513..25217 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
BWI86_RS25725 25285..26583 + 1299 Protein_30 Tn3-like element Tn3 family transposase -
BWI86_RS25730 26617..27117 + 501 Protein_31 Tn3 family transposase -
BWI86_RS25735 27151..27339 + 189 Protein_32 IS3 family transposase -
BWI86_RS25740 27414..27701 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BWI86_RS25745 27698..27949 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BWI86_RS25760 28917..29771 - 855 Protein_35 incFII family plasmid replication initiator RepA -
BWI86_RS25765 29764..29838 - 75 WP_061357229.1 RepA leader peptide Tap -
BWI86_RS25775 30070..30327 - 258 WP_001541895.1 replication regulatory protein RepA -
BWI86_RS25780 30595..30801 - 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
- 30788..30849 + 62 NuclAT_1 - -
- 30788..30849 + 62 NuclAT_1 - -
- 30788..30849 + 62 NuclAT_1 - -
- 30788..30849 + 62 NuclAT_1 - -
BWI86_RS25790 31191..31403 - 213 WP_001541896.1 ANR family transcriptional regulator -
BWI86_RS25795 31538..32098 - 561 WP_000139310.1 fertility inhibition protein FinO -
BWI86_RS25800 32153..32896 - 744 WP_039002216.1 conjugal transfer pilus acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / sul2 / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / ant(3'')-Ia / aac(3)-IId / tet(M) / cmlA1 / aadA2 / dfrA12 - 1..138610 138610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T72051 WP_000222766.1 NZ_CP019261:c27701-27414 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T72051 NZ_CP019261:c27701-27414 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT72051 WP_001132900.1 NZ_CP019261:c27949-27698 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT72051 NZ_CP019261:c27949-27698 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References